Research Catalog

Introduction to immunocytochemistry

Title
Introduction to immunocytochemistry / J.M. Polak, S. Van Noorden.
Author
Polak, Julia M.
Publication
Oxford : BIOS Scientific ; New York : Springer, in association with the Royal Microscopical Society, c1997.

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Details

Additional Authors
Van Noorden, Susan.
Description
xiv, 141 p. : ill. (some col.); 24 cm.
Series Statement
Royal Microscopial Society microscopy handbooks ; 37
Uniform Title
Microscopy handbooks ; 37.
Subject
Bibliography (note)
  • Includes bibliographical references and index.
Contents
Introduction -- Production of antibodies -- Requirements for immunocytochemistry -- Colour plates -- Methods -- Specificity problems and essential controls -- Enhancement of standard methods -- Multiple immunostaining -- Post-embedding immunocytochemistry for the transmission electron microscope -- In vitro methods for testing antigen-antibody reactions -- Applications of immunocytochemistry -- Microscopy -- Appendix: technical notes -- Buffers -- Antibody diluent and storage of antibodies -- Adherence of preparations to slides -- Blocking endogenous peroxidase reaction -- Enzyme pre-treatment -- Heat-mediated antigen retrieval using a microwave oven -- Enzyme development methods -- Intensifying the peroxidase/DAB reaction product -- Immunostaining methods -- Immunogold staining with silver enhancement -- Double immunoenzymatic staining -- Post-embedding electron microscopical -- Absorption specificity control (liquid phase) -- References -- Index.
Call Number
JSE 97-1460
ISBN
0387915133 (softcover : alk. paper)
LCCN
97013062
OCLC
36676060
Author
Polak, Julia M.
Title
Introduction to immunocytochemistry / J.M. Polak, S. Van Noorden.
Imprint
Oxford : BIOS Scientific ; New York : Springer, in association with the Royal Microscopical Society, c1997.
Edition
2nd ed.
Series
Royal Microscopial Society microscopy handbooks ; 37
Microscopy handbooks ; 37.
Bibliography
Includes bibliographical references and index.
Added Author
Van Noorden, Susan.
Research Call Number
JSE 97-1460
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