Research Catalog

Title

Planar lipid bilayers : methods and applications / W. Hanke, W.-R. Schlue.

Author

Hanke, W.

Publication

London ; New York : Academic Press, [1993], ©1993.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Schlue, W.-R.

Description

x, 133 pages : illustrations; 26 cm.

Series Statement

Biological techniques series

Uniform Title

Biological techniques series.

Subject

Lipid Bilayers

Bibliography (note)

• Includes bibliographical references and index.

Contents

• Ch. 1. Introduction. 1.1. Planar lipid bilayers: tools for investigating biological membranes. 1.2. The patch-clamp technique -- Ch. 2. Physical properties of biological membranes and planar lipid bilayers. 2.1. Physical properties of pure lipid membranes. 2.2. Proteins in lipid membranes. 2.3. Transport across membranes -- Ch. 3. Technical details of bilayer experiments. 3.1. Mechanics. 3.2. Optics. 3.3. Electronics. 3.4. Data acquisition and computer support. 3.5. Additional equipment -- Ch. 4. Biochemical preparations for planar lipid bilayer experiments. 4.1. Lipids. 4.2. Proteins. 4.3. How to make large liposomes for patch-clamp experiments. 4.4. Flux measurements with liposomes -- Ch. 5. Methods for making planar lipid bilayers. 5.1. Painted lipid bilayers. 5.2. Folded bilayers. 5.3. Dip-stick bilayers. 5.4. Patch-clamping of large liposomes -- Ch. 6. Incorporation of proteins into planar lipid bilayers. 6.1. Water-soluble compounds. 6.2. Membrane proteins. 6.3. Pipette techniques.
• 6.4. Attachment of membrane fragments to pre-formed bilayers -- Ch. 7. Data acquisition and evaluation. 7.1. Structure and types of data obtained. 7.2. Preliminary data evaluation by eye and hand. 7.3. Data evaluation using computers. 7.4. General comments -- Ch. 8. Advanced experiments and examples of data. 8.1. How to control the physical properties of planar lipid bilayers. 8.2. Investigation of the alamethicin pore. 8.3. Reconstitution of the nicotinic acetylcholine receptor channel. 8.4. Further examples of transport proteins investigated in planar lipid bilayer experiments. 8.5. What can be learned about protein structure and function from bilayer experiments. 8.6. Bilayer experiments as tools for functional purification of ion channel proteins. 8.7. Optical measurements on planar lipid bilayers. 8.8. Study of membrane fusion with planar lipid bilayers. 8.9. Lipid dependency of ion channel parameters -- App. 1 List of symbols and abbreviations used -- App. 2 Letter code for amino acids.
• App. 3 List of useful constants, data and equations -- App. 4 Sources of supply -- App. 5 Key references.

ISBN

• 0123229944 (hardback)
• 0123229952 (bench-top edition)

OCLC

ocm29742461

Owning Institutions

Columbia University Libraries