Research Catalog
YAC libraries : a user's guide
- Title
- YAC libraries : a user's guide / edited by David L. Nelson, Bernard H. Brownstein.
- Publication
- New York : W.H. Freeman and Co., [1994], ©1994.
Items in the Library & Off-site
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1 Item
| Status | Format | Access | Call Number | Item Location |
|---|---|---|---|---|
| Text | Request in advance | TP248.27.Y43 Y33 1994 | Off-site |
Holdings
Details
- Additional Authors
- Description
- xx, 211 pages : illustrations; 23 cm.
- Series Statement
- UWBC biotechnical resource series
- Uniform Title
- UWBC biotechnical resource series.
- Subjects
- Genre/Form
- Catalog.
- Bibliography (note)
- Includes bibliographical references and index.
- Contents
- 1. Vectors and Host Strains for Cloning and Modification of Yeast Artificial Chromosomes -- 2. Yeast Spheroplast Transformation with Large DNA -- 3. Large Insert Yeast Artificial Chromosome Libraries -- 4. Isolation of YAC Clones from Genomic Libraries by Southern Blot Hybridization -- 5. Generation of YAC Contigs by Walking -- 6. The Use of YACs to Identify Expressed Sequences: cDNA Screening Using Total YAC Insert -- 7. YAC Clones as an Integral Component in the Positional Cloning of Human Disease Genes: Isolation of the APC Gene -- 8. Transfer of YAC DNA into Mammalian Cells and Transgenic Mice -- 9. Fluorescent in Situ Hybridization to Metaphase Chromosomes with YACs -- 10. YAC Breeding to Reconstitute Large Loci -- Appendix: Growth Media.
- ISBN
- 0716770148
- LCCN
- 93013780
- OCLC
- ocm28148592
- Owning Institutions
- Columbia University Libraries