Research Catalog

Title

Protocols for nucleic acid analysis by nonradioactive probes / edited by Peter G. Isaac.

Publication

Totowa, N.J. : Humana Press, [1994], ©1994.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Isaac, Peter G.

Description

xii, 268 pages : illustrations; 23 cm.

Series Statement

Methods in molecular biology ; 28

Uniform Title

Methods in molecular biology (Clifton, N.J.) ; v. 28.

Alternative Title

Nonradioactive probes.

Subject

• Nucleic acid probes
• Nucleic acids > Analysis

Bibliography (note)

• Includes bibliographical references and index.

Contents

• Ch. 1. About Nonradioactive Nucleic Acid Detection / Peter G. Isaac -- Ch. 2. Isolation of DNA from Plants / Justin Stacey and Peter G. Isaac -- Ch. 3. Isolation of High-Molecular-Weight DNA from Animal Cells / Ian Garner -- Ch. 4. Restriction Enzyme Digestion, Gel Electrophoresis, and Vacuum Blotting of DNA to Nylon Membranes / Justin Stacey and Peter G. Isaac -- Ch. 5. Isolation of Plant RNA / Rachel Hodge -- Ch. 6. Isolation of Total and PolyA[superscript +] RNA from Animal Cells / Ian Garner -- Ch. 7. Preparation of RNA Gel Blots / Rachel Hodge -- Ch. 8. Preparation of RNA Dot Blots / Rachel Hodge -- Ch. 9. Isolation of Plasmids for the Preparation of Probes / Peter G. Isaac and Justin Stacey -- Ch. 10. Production of Hybridization Probes by the PCR Utilizing Digoxigenin-Modified Nucleotides / Tom McCreery and Tim Helentjaris -- Ch. 11. Production of DNA Hybridization Probes with Digoxigenin-Modified Nucleotides by Random Hexanucleotide Priming / Tom McCreery and Tim Helentjaris.
• Ch. 12. Digoxigenin Labeling of RNA Transcripts from Multi- and Single-Locus DNA Minisatellite Probes / Esther N. Signer -- Ch. 13. Labeling of Double-Stranded DNA Probes with Biotin / Angela Karp -- Ch. 14. Preparation of Horseradish Peroxidase-Labeled Probes / Ian Durrant and Timothy Stone -- Ch. 15. Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP / Bronwen M. Harvey, Claire B. Wheeler and Martin W. Cunningham -- Ch. 16. Labeling of Oligonucleotides with Fluorescein / Patricia M. E. Chadwick and Ian Durrant -- Ch. 17. Hybridization of Digoxigenin-Labeled Probes to Southern Blots and Detection by Chemiluminescence / Tom McCreery and Tim Helentjaris -- Ch. 18. Southern Blot Hybridization of Digoxigenin-Labeled RNA Minisatellite Probes and Color Detection / Esther N. Signer -- Ch. 19. Hybridization and Detection of Digoxigenin Probes on RNA Blots / Elizabeth Davies, Rachel Hodge and Peter G. Isaac.
• Ch. 20. Hybridization of Horseradish Peroxidase-Labeled Probes and Detection by Enhanced Chemiluminescence / Timothy Stone and Ian Durrant -- Ch. 21. Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Enhanced Chemiluminescence / Bronwen M. Harvey and Claire B. Wheeler -- Ch. 22. Hybridization of Fluorescein-Labeled Oligonucleotide Probes and Enhanced Chemiluminescence Detection / Ian Durrant and Patricia M. E. Chadwick -- Ch. 23. Preparation of Chromosome Spreads by Root-Tip Meristem Dissection for In Situ Hybridization with Biotin-Labeled Probes / Angela Karp -- Ch. 24. Enzymatic Treatment of Plant Material to Spread Chromosomes for In Situ Hybridization / Trude Schwarzacher and Andrew R. Leitch -- Ch. 25. Use of Biotin-Labeled Probes on Plant Chromosomes / Angela Karp -- Ch. 26. Direct Fluorochrome-Labeled DNA Probes for Direct Fluorescent In Situ Hybridization to Chromosomes / Trude Schwarzacher and J. S. (Pat) Heslop-Harrison.
• Ch. 27. Detection of Digoxigenin-Labeled DNA Probes Hybridized to Plant Chromosomes In Situ / Ilia J. Leitch and J. S. (Pat) Heslop-Harrison -- Ch. 28. Preparation of Tissue Sections and Slides for mRNA Hybridization / Giorgio Terenghi and Julia M. Polak -- Ch. 29. Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes / Giorgio Terenghi and Julia M. Polak -- Ch. 30. Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes / Barry Rosen and Rosa Beddington -- Ch. 31. PACE (Probe Assay - Chemiluminescence Enhanced): A Magnetic Bead Assay for Noncultural Diagnosis of Gonorrhea / Paul A. Granato -- Ch. 32. Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format / E. Patrick Groody -- Ch. 33. Analysis of Gene Sequences by Hybridization of PCR-Amplified DNA to Covalently Bound Oligonucleotide Probes: The Reverse Dot Blot Method / Ernest S. Kawasaki and Farid F. Chehab.
• Ch. 34. RAPD Assay: A Novel Technique for Genetic Diagnostics / Joseph P. del Tufo and Scott V. Tingey -- Ch. 35. Nonradioactive Oligonucleotide Probes for Detecting Products of the Ligase Chain Reaction / Jon Kratochvil and Thomas G. Laffler -- Ch. 36. Nucleic Acid Sequence-Based Amplification (NASBA) / Larry Malek, Roy Sooknanan and Jean Compton.

ISBN

089603254X

LCCN

93029390

OCLC

• 28631413
• ocm28631413

Owning Institutions

Columbia University Libraries