Research Catalog

Title

Protein phosphorylation : a practical approach / edited by D. Grahame Hardie.

Publication

Oxford ; New York : Oxford University Press, [1993], ©1993.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Hardie, D. G. (D. Grahame)

Description

xxii, 300 pages : illustrations; 25 cm.

Summary

• Reversible phosphorylation is one of the major mechanisms by which protein activity is controlled in all eukaryotic cells. Networks of protein kinases and phosphatases, which catalyse phosphorylation and dephosphorylation, respectively, are responsible for switching between different pathways of cell division, development, or differentiation in response to hormones or growth factors.
• This book provides a comprehensive description of current methods for studying protein phosphorylation and the protein kinases and phosphatases which catalyse it. It includes detailed protocols for studying phosphorylation in intact cells, approaches using enzyme inhibitors, purification of serine- and threonine-specific protein kinases and phosphatases, analysis of kinase and phosphatase specificity, and cloning relevant cDNAs.
• . Protein Phosphorylation: A Practical Approach will be of great value to all researchers in the fields of intracellular and intercellular signalling, including biochemists, molecular biologists, and pharmacologists.

Series Statement

Practical approach series

Uniform Title

Practical approach series.

Subject

• Phosphoproteins > Methodology
• Phosphorylation > Methodology
• Protein kinases > Methodology
• Phosphoprotein phosphatases > Methodology
• Phosphorylation
• Protein Kinases
• Proteins

Bibliography (note)

• Includes bibliographical references and index.

Contents

• 1. Study of protein phosphorylation in intact cells / James C. Garrison -- 2. Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates / Peter van der Geer, Kunxin Luo, Bartholomew M. Sefton and Tony Hunter -- 3. Analysis of sites phosphorylated in vivo and in vitro / D. Grahame Hardie, David G. Campbell, F. Barry Caudwell and Timothy A. J. Haystead -- 4. Use of protein (serine/threonine) kinase activators and inhibitors to study protein phosphorylation in intact cells / Hiroyoshi Hidaka and Ryoji Kobayashi -- 5. Use of protein phosphatase inhibitors in intact cells / D. Grahame Hardie -- 6. Purification and assay of mammalian protein (serine/threonine) kinases / Yuhuan Wang and Peter J. Roach -- 7. Purification of protein (tyrosine) kinases / Alexander A. Stewart -- 8. Homology-based approaches for identifying cDNAs that encode eukaryotic protein (serine/threonine) and protein (tyrosine) kinases / Steven Hanks and Michael A. Lawton.
• 9. Assay and purification of protein (serine/threonine) phosphatases / Carol MacKintosh -- 10. Characterization of protein (tyrosine) phosphatases / Nicholas K. Tonks -- 11. Identification of novel protein (serine/threonine) phosphatase genes in Saccharomyces cerevisiae, Drosophila melanogaster, and man by the polymerase chain reaction / Mao Xiang Chen and Patricia T. W. Cohen -- 12. Studies of protein kinase/phosphatase specificity using synthetic peptides / Richard B. Pearson, Kenneth I. Mitchelhill and Bruce E. Kemp -- Appendix: Suppliers of specialist items.

ISBN

• 0199633061 (hbk.) :
• 0199633053 (pbk.) :

LCCN

92048506

OCLC

ocm27311140

Owning Institutions

Columbia University Libraries