Research Catalog

Title

Heterotrimeric G protein effectors / edited by Ravi Iyengar.

Publication

San Diego : Academic Press, [1994], ©1994.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Iyengar, Ravi.

Description

xxix, 456 pages : illustrations; 24 cm.

Series Statement

Methods in enzymology, 0076-6879 ; v. 238

Uniform Title

Methods in enzymology ; v. 238.

Subjects

• GTP-Binding Proteins
• Enzymes > Regulation
• Signal Transduction
• G proteins

Bibliography (note)

• Includes bibliographical references and index.

Contents

• 1. Purification of Bovine Retinal cGMP Phosphodiesterase / Andrea Tar, Tuow D. Ting and Yee-Kin Ho -- 2. Specific Peptide Probes for G-Protein Interaction with Effectors / Helen M. Rarick, Nikolai O. Artemyev, John S. Mills, Nikolai P. Skiba and Heidi E. Hamm -- 3. Determination of Adenylyl Cyclase Catalytic Activity Using Single and Double Column Procedures / Roger A. Johnson, Robert Alvarez and Yoram Salomon -- 4. Preparation and Use of "P"-Site-Targeted Affinity Ligands for Adenylyl Cyclases / Roger A. Johnson and Ilana Shoshani -- 5. Regulation of Ca2f-Sensitive Adenylyl Cyclases by Calcium in Vitro and in Vivo / Dermot M. F. Cooper -- 6. G[subscript i] Assays in Transfected Cells / Yung H. Wong -- 7. Expression and Purification of Recombinant Adenylyl Cyclases in Sf9 Cells / Ronald Taussig, Wei-Jen Tang and Alfred G. Gilman -- 8. Transient Expression Assays for Mammalian Adenylyl Cyclases / Ofer Jacobowitz, Jianqiang Chen and Ravi Iyengar.
• 9. Identification of Adenylyl Cyclases by Amplification Using Degenerate Primers / Richard T. Premont -- 10. Assays for G-Protein Regulation of Phospholipase C Activity / Michael De Vivo -- 11. Chloride Current Assay for Phospholipase C in Xenopus Oocytes / Emmanuel M. Landau and Robert D. Blitzer -- 12. Use of Cytosol-Depleted HL60 Cells for Reconstitution Studies of G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-[beta] Isozymes / Shamshad Cockcroft, Geraint M. H. Thomas, Emer Cunningham and Andrew Ball -- 13. Purification of Phosphatidylinositol Transfer Protein from Brain Cytosol for Reconstituting G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-[beta] Isozymes / Geraint M. H. Thomas, Emer Cunningham and Shamshad Cockcroft -- 14. Stimulation of Phospholipase C by G-Protein [beta] [gamma] Subunits / Peter Gierschik and Montserrat Camps.
• 15. Purification of G-Protein-Regulated Phospholipase C from Turkey Erythrocytes / Gary L. Waldo, Andrew J. Morris and T. Kendall Harden -- 16. Phospholipase C Activity in Dictyostelium discoideum Using Endogenous Nonradioactive Phosphatidylinositol 4,5-Bisphosphate as Substrate / Anthony A. Bominaar and Peter J. M. Van Haastert -- 17. Purification of Phospholipase C-[beta]3 from Rat Brain / Deok-Young Jhon, Dongeun Park and Sue Goo Rhee -- 18. Characterization of Phospholipase C Isozymes in Bovine Retina: Purification of Phospholipase C-[beta]4 / Chang-Won Lee, Kweon-Haeng Lee and Sue Goo Rhee -- 19. Purification of 110 kDa Phosphoinositide Phospholipase C Activated by G-Protein [beta] [gamma] Subunits / J. L. Blank and J. H. Exton -- 20. Amplification of Phosphatidylinositol-Specific Phospholipase C-[beta] Isoforms Using Degenerate Primers / Hai-Wen Ma, Ravi Iyengar and Richard T. Premont.
• 21. Analysis of Guanine Nucleotides Associated with Protooncogene Ras / Richard R. Vaillancourt, Anne E. Harwood and Sim Winitz -- 22. Measuring Activation of Kinases in Mitogen-Activated Protein Kinase Regulatory Network / Anne M. Gardner, Carol A. Lange-Carter, Richard R. Vaillancourt and Gary L. Johnson -- 23. Transcriptional Activation Analysis of Oncogene Function / Craig A. Hauser, Channing J. Der and Adrienne D. Cox -- 24. Biological Assays for Cellular Transformation / Adrienne D. Cox and Channing J. Der -- 25. Monitoring of Receptor-Mediated Changes in Intracellular Calcium at the Cellular and Subcellular Levels by Microfluorimetry and Imaging / Stephen R. Rawlings, Jean-Marc Theler and Werner Schlegel -- 26. Combination of Microfluorimetric Monitoring of Cytosolic Calcium and pH with Patch Clamp Electrophysiological Recordings in Neutrophil Granulocytes / Nicolas Demaurex, Stephen R. Rawlings, Karl-Heinz Krause, Marisa E. E. Jaconi, P. Daniel Lew and Werner Schlegel.
• 27. G-Protein-Mediated Pathways Assayed by Electrophysiology and Confocal Microscopy / Lisa Stehno-Bittel, Jeff Amundson and David E. Clapham -- 28. Purification and Reconstitution of N-Type Calcium Channel Complex from Rabbit Brain / Derrick R. Witcher, Michel De Waard, Steven D. Kahl and Kevin P. Campbell -- 29. Infusion of Guanine Nucleotides through Recording Electrodes for Studies on G-Protein Regulation of Ion Currents and Channels / Rodrigo Andrade -- 30. Injection of Antisera into Cells to Study G-Protein Regulation of Channel Function / I. McFadzean, M. P. Caulfield, Y. Vallis and D. A. Brown -- 31. Whole-Cell Clamp Analysis for G-Protein Regulation of Channels / J. Hescheler -- 32. Whole-Cell Patch Recording in Brain Slices / Robert D. Blitzer and Emmanuel M. Landau -- 33. Reconstitution of Receptor-Regulated Ion Channels in Isolated Patch Membrane / Atsuko Yatani.
• 34. Regulation of Potassium Channels by G-Protein Subunits and Arachidonic Acid Metabolites / Mitsuhiko Yamada, Andre Terzic and Yoshihisa Kurachi.

ISBN

0121821390

OCLC

• 31517827
• ocm31517827

Owning Institutions

Columbia University Libraries