Research Catalog

Plant tissue culture : techniques and experiments

Title
Plant tissue culture : techniques and experiments / by Roberta H. Smith.
Author
Smith, Roberta H.
Publication
San Diego : Academic Press, [1992], ©1992.

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Description
xvii, 171 pages : illustrations; 24 cm
Subject
Bibliography (note)
  • Includes bibliographical references and index.
Contents
  • 1. Setup of a Tissue Culture Laboratory. Work Areas -- 2. Media Components and Preparation. Inorganic Salts. Plant Growth Regulators. Vitamins. Carbohydrates. Hexitols. Gelling Agent. Amino Acids. Antibiotics. Natural Complexes -- 3. Explant Preparation. Aseptic Technique. Aseptic Germination of Seeds -- 4. Callus Induction. Callus Initiation. Explant Orientation. Establishment of Competent Cereal Cell Cultures (Rice). Salt Selection in Vitro. Growth Curves. Cellular Variation from Callus Cultures -- 5. Regeneration and Morphogenesis. Controlled Morphogenesis: Potato Tuberization. Somatic Embryogenesis. Regeneration of Rice. Dormancy Requirements of Explants. Primary Morphogenesis: Douglas Fir -- 6. Haploid Plants from Anther Culture. Datura. African Violet. Tobacco. Anther Squash Technique -- 7. Embryo Rescue. Sweet Corn. Crabapple and Pear -- 8. Meristem Culture for Virus-Free Plants. Isolation of Shoot Apical Meristem. Establishing Virus- and Bacteria-Free Plants.
  • Garlic Propagation from Bulb Scales -- 9. In Vitro Propagation for Commercial Production of Ornamentals. Boston Fern. Staghorn Fern: Stages II and III. Ficus: Stages I, II, and III. Kalanchoe. Rose. African Violet. Endangered Species -- 10. Protoplast Isolation and Fusion -- 11. Agrobacterium-Mediated Transformation of Plants. Petunia Leaf Disk. Petunia Shoot Apex -- 12. Useful Measurements -- 13. Review of Solution Preparation. Percentage Solutions. Molar Solutions. Dilution of a Stock Solution -- 14. List of Suppliers -- 15. Common Plant Tissue Culture Terms.
ISBN
0126503400 (acid-free paper)
LCCN
92233094
OCLC
  • 25281335
  • ocm25281335
Owning Institutions
Columbia University Libraries