Research Catalog

Title

Practical flow cytometry / Howard M. Shapiro.

Author

Shapiro, Howard M. (Howard Maurice), 1941-

Publication

New York : Wiley-Liss, 1995.

Items in the Library & Off-site

Details

Description

xxxviii, 542 pages, 8 unnumbered pages of plates : illustrations (some color); 29 cm

Subjects

• Flow cytometry
• Flow Cytometry

Bibliography (note)

• Includes bibliographical references (p. 445-516) and index.

Contents

• Foreword to the Third Edition / Leonard A. Herzenberg -- Foreword to the First Edition / Louis A. Kamentsky -- 1. Overture. 1.1. What Is Flow Cytometry? 1.2. Flow Cytometry And Microscopy. 1.3. Flow Cytometer Anatomy In Brief. 1.4. Beyond Positive And Negative: Putting The -Metry In Flow Cytometry. 1.5. Flow Cytometry In The 1990's. 1.6. The Rest Of The Book -- 2. Learning Flow Cytometry. 2.1. Flow Cytometry: General References. 2.2. The Reader's Guide To Periodical Literature. 2.3. Resources And Courses. 2.4. Exploring The Foundations. 2.5. Alternatives To Flow Cytometry -- 3. History. 3.1. Ancient History. 3.2. Classical History. 3.3. Modern History -- 4. How Flow Cytometers Work. 4.1. Light and Matter. 4.2. Optical Systems. 4.3. Light Sources. 4.4. Light Collection. 4.5. Detectors. 4.6. Flow Systems. 4.7. Electronic Measurements. 4.8. Signal Processing. 4.9. Linear, Log and Ratio Circuits. 4.10. Precision, Sensitivity, and Accuracy -- 5. Data Analysis. 5.1. Goals and Methods in Data Analysis.
• 5.2. Analog-To-Digital Conversion. 5.3. Computer Systems for Flow Cytometry. 5.4. Primary Data: Frequency Distributions. 5.5. Multiparameter Data Analysis. 5.6. Compensating Without Decompensating. 5.7. Analysis of Collected Data: How Much Is Enough/Too Much? 5.8. Data Storage -- 6. Flow Sorting. 6.1. Sort Control (Decision) Logic. 6.2. Preselected Count Circuits and Single Cell Sorting. 6.3. Droplet Sorting. 6.4. Fluidic Switching Cell Sorters. 6.5. Cell Manipulation By Optical Trapping. 6.6. Cell Damage Cell Selection ("Cell Zapping"). 6.7. Measures of Cell Sorter Performance: Purity, Recovery (Yield), and Efficiency. 6.8. Other Considerations. 6.9. Conclusions.

ISBN

0471303763

LCCN

94030756

OCLC

• 30914270
• ocm30914270

Owning Institutions

Columbia University Libraries