Research Catalog

Title

Genetic analysis ; principles, scope, and abjectives / John R.S. Fincham.

Author

Fincham, J. R. S.

Publication

Oxford ; Boston : Blackwell Scientific Publications, 1994.

Items in the Library & Off-site

Holdings

Details

Description

xi, 227 pages : illustrations; 25 cm

Subjects

• Sequence Analysis, DNA
• Gene mapping
• Genetics > Technique
• Genome
• Nucleotide sequence

Bibliography (note)

• Includes bibliographical references and index.

Contents

• Introduction: the expanding scope of genetics -- 1. Dissecting the genome using natural genetic systems. The eukaryotic system. Meiosis and the rules of classical genetics. Genetic analysis of bacteria and bacteriophage. Eukaryotic organelle genetics -- 2. From mutations to genes. Defining the gene by mutation and complementation. Mapping within the gene. The determination by genes of protein structure -- 3. The gene as DNA sequence. Characterization of DNA fragments. Cloning and cloning vectors. Screening DNA libraries for functional genes. Confirming the identity of cloned sequences. Simultaneous mutagenesis and gene tagging -- 4. The evolving concept of the gene. The gene as a cis-acting unit. The gene as a unit of translation. The gene as a unit of transcription. Processing of the primary transcript. The complex functions of promoters. Enhancers - the expanding frontiers of the gene. Restructured genes and edited messages -- 5. Analysis of the whole genome.
• Total DNA - quantities and patterns of complexity. The nature of repetitive DNA. Chromosome characterization by microscopy. Chromosome sorting - chromosome-specific probes and libraries. Comprehensive genetic mapping with molecular markers. From physical map to complete DNA sequence. DNA of the organelles -- Conclusion: total analysis of genomes - what benefits to understanding? -- 6. Accounting for heritable variation. Molecular identification of mutations of strong effect. DNA sequence variation without phenotypic consequences. Quantitative variation and its heritability. Attributing heritable quantitative variation to chromosomal loci -- 7. Gene interactions and the genetic programme. Examples from the yeasts. Another level of control - RNA splicing in Drosophila sexual development. The emergence of morphological pattern. Selector genes in Drosophila and other organisms. Timing of gene expression - a function for introns? Stabilizing gene activity - epigenetic effects.
• Towards the complete description of the organism.

ISBN

0632036591

LCCN

94008646

OCLC

• 30035737
• ocm30035737

Owning Institutions

Columbia University Libraries