Research Catalog

Somatic cell hybrids : the basics

Title
Somatic cell hybrids : the basics / C. Abbott and S. Povey.
Author
Abbott, C.
Publication
Oxford ; New York : Oxford University Press, 1994.

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StatusFormatAccessCall NumberItem Location
TextRequest in advance QH451 .A22 1995Off-site

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Additional Authors
Povey, S. (Sue)
Description
ix, 76 pages : illustrations; 1995.
Subjects
Bibliography (note)
  • Includes bibliographical references and index.
Contents
  • Ch. 1. Why do we map genes? 1. What are somatic cell hybrids? 2. Uses of somatic cell hybrids. 3. How do we map genes? 4. How else can somatic cell hybrids be of use? 5. Where do I begin? 6. Where do I find out more? -- Ch. 2. Making somatic cell hybrids. 1. Characteristics of different cultured cells. 2. Selection of hybrid cells. 3. Making a hybrid panel -- Ch. 3. Characterizing somatic cell hybrids -- 1. Introduction. 2. Karyotyping. 3. Isoenzymes. 4. Southern blotting. 5. Polymerase chain reaction (PCR) -- Ch. 4. Mapping genes using somatic cell hybrids -- 1. Introduction: the concepts of mapping panel. 2. Which technique to use for mapping. 3. Some problems commonly encountered -- Ch. 5. Modification of the basic techniques - designer hybrids. 1. Selection of chromosomes other than X or 17. 2. Hybrids containing single chromosomes. 3. Hybrids containing parts of chromosomes -- Ch. 6. Other uses of hybrids. 1. Direct isolation of disease genes. 2. Generation of region-specific probes from hybrids.
  • 3. Differentiation. 4. Malignancy. 5. Hybridomas.
ISBN
0199634432 :
LCCN
94019265
OCLC
  • 30546279
  • ocm30546279
Owning Institutions
Columbia University Libraries