Research Catalog
Immunochemistry in practice
- Title
- Immunochemistry in practice / Alan Johnstone, Robin Thorpe.
- Author
- Johnstone, Alan.
- Publication
- Oxford ; Cambridge, Mass. : Blackwell Science, 1996.
Items in the Library & Off-site
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1 Item
| Status | Format | Access | Call Number | Item Location |
|---|---|---|---|---|
| Text | Request in advance | QR183.6 .J63 1996 | Off-site |
Details
- Additional Authors
- Thorpe, Robin.
- Description
- xv, 362 pages : illustrations (some color); 25 cm
- Summary
- Immunochemical techniques are invaluable in all biological disciplines for detecting, estimating and isolating molecules and cells. The aim of this practical book is to provide scientists, whatever their specialty, with clear step-by-step details of immunochemical procedures. This third edition of Immunochemistry in Practice has been completely revised and updated; some chapters have been restructured and there are several new sections, a new chapter and many new illustrations.
- A revised layout has been adopted for improved ease of use.
- Subject
- Genre/Form
- Laboratory Manual.
- Bibliography (note)
- Includes bibliographical references (p. 347-353) and index.
- Contents
- 1. Basic techniques -- 2. Production of antibodies -- 3. Purification of immunoglobulins, constituent chains and fragments -- 4. Isolation and fractionation of lymphocytes -- 5. Radiolabelling techniques -- 6. Precipitation techniques in agar and agarose -- 7. Analytical electrophoresis and isoelectric focusing -- 8. Specific detection of antigens separated by polyacrylamide gel electrophoresis -- 9. Preparative gel techniques -- 10. Affinity chromatography and immunoprecipitation -- 11. Coupling antibodies to fluorochromes, enzymes and biotin -- 12. Immunoassays -- 13. Immunocytochemistry, immunohistochemistry and flow cytofluorimetry -- Appendix 2: Buffers -- Appendix 3: Manufacturers and suppliers.
- ISBN
- 0865426333
- LCCN
- 95039026
- OCLC
- ocm33166514
- Owning Institutions
- Columbia University Libraries