Research Catalog

Lipases.

Title
Lipases. Part B, Enzyme characterization and utilization / edited by Byron Rubin, Edward A. Dennis.
Publication
San Diego : Academic Press, [1997], ©1997.

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TextRequest in advance QP601 .C71 v.286Off-site

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Description
xxxi, 563 pages : illustrations; 24 cm.
Series Statement
Methods in enzymology ; v. 286
Uniform Title
Methods in enzymology ; v. 286.
Subjects
Bibliography (note)
  • Includes bibliographical references and indexes.
Contents
Sect.1. Regulation and characterization -- Inhibition of lipid absorption as an approach to the treatment of obesity -- Regulation of hormone-sensitive lipase activity in adipose tissue -- Use of gene knockout mice to establish lipase function -- Properties of pancreatic carboxyl ester lipase in mRNA-injected Xenopus oocytes and transfected mammalian hepatic and intestinal cells -- Noncatalytic functions of lipoprotein lipase -- Radiation inactivation studies of hepatic cholesteryl ester hydrolases -- Immunological techniques for the characterization of digestive lipases -- Sect.2. Substrate and inhibitor characterization -- Physical behavior of lipase substrates -- Phospholipase A₂ activity and physical properties of lipid-bilayer substances -- Covalent inactivation of lipases -- Mechanism-based inhibitors of mammalian cholesterol esterase -- On the inhibition of microbial lipases by tetrahydrolipstatin -- Monolayer techniques for studying lipase kinetics -- Recovery of monomolecular films in studies of lipolysis -- Oil-drop tensiometer: applications for studying the kinetics of lipase action -- A critical reevaluation of the phenomenon of interfacial activation -- Sect.3. Biocatalytic utility -- Screening techniques for lipase catalyst selection -- Kinetics, molecular modeling, and synthetic applications with microbial lipases -- Ester synthesis via acyl transfer (transesterification) -- Cross-linked enzyme crystals of lipases as catalysts for kinetic resolution of acids and alcohols -- Measurement and control of hydration in non-aqueous biocatalysis -- Solvent effect in lipase-catalyzed racemate resolution -- Lipases in supercritical fluids -- Stabilization of lipases against deactivation of acetaldehyde formed in acyl transfer reactions.
ISBN
0121821870
OCLC
ocm37650066
Owning Institutions
Columbia University Libraries