Research Catalog

Title

The yeast two-hybrid system / edited by Paul L Bartel & Stanley Fields.

Publication

New York : Oxford University Press, 1997.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Bartel, Paul L.
• Fields, Stanley.

Description

xi, 344 pages : illustrations; 24 cm.

Summary

• This volume, part of the Advances in Molecular Biology series, presents work by pioneers in the field and is the first publication devoted solely to the yeast two-hybrid system. It includes detailed protocols, practical advice on troubleshooting, and suggestions for future development. In addition, it illustrates how to construct an activation domain hybrid library, how to identify mutations that disrupt an interaction, and how to use the system in mammalian cells.
• Many of the contributors have developed new applications and variations of the technique.

Series Statement

Advances in molecular biology

Uniform Title

Advances in molecular biology.

Subjects

• Plasmids
• Yeast fungi
• Protein binding > Research > Methodology

Bibliography (note)

• Includes bibliographical references and index.

Contents

• 1. The Two-Hybrid System: A Personal View / Stanley Fields and Paul L. Bartel -- 2. Searching for Interacting Proteins with the Two-Hybrid System I / Chang Bai and Stephen J. Elledge -- 3. Searching for Interacting Proteins with the Two-Hybrid System II / Anne B. Vojtek, Jonathan A. Cooper and Stanley M. Hollenberg -- 4. Searching for Interacting Proteins with the Two-Hybrid System III / Erica A. Golemis and Roger Brent -- 5. Constructing an Activation Domain-Fusion Library / Li Zhu, David Gunn and Sailaja Kuchibhatla -- 6. Obtaining Structural Information about Protein Complexes with the Two-Hybrid System / David C. Amberg and David Botstein -- 7. The Reverse Two-Hybrid System / Marc Vidal -- 8. Dissecting the Biological Relevance of Multiple Interactions / Michael A. White -- 9. Investigation of Ligand/Receptor Interactions and the Formation of Tertiary Complexes / Bradley A. Ozenberger and Kathleen H. Young --
• 10. Two-Hybrid Analysis of Protein-Protein Interactions in the Yeast Pheromone Response Pathway / George F. Sprague, Jr. and John A. Printen -- 11. Two-Hybrid Screening and the Cell Cycle / George J. Hannon -- 12. Two-Hybrid Analysis of Genetic Regulatory Networks / Russell L. Finley, Jr. and Roger Brent -- 13. Mammalian Cells as Hosts for Two-Hybrid Studies of Protein-Protein Interaction / Julia Tsou Tsan, Zhuouel Wang and Ying Jin [et al.] -- 14. Detection of Protein-Protein Interactions Dependent on Post-Translational Modifications / Mark A. Osborne, Manuel Lubinus and Jarema P. Kochan -- 15. Protein Interactions Mediated by Small Molecule Ligands / Vivian Berlin -- 16. Use of a Combinatorial Peptide Library in the Two-Hybrid Assay / Meijia Yang -- 17. Isolation of DNA-Binding Proteins Using a One-Hybrid Genetic Screen / Jayhong A. Chong and Gail Mandel --
• 18. A Three-Hybrid to Detect and Analyze RNA-Protein Interactions In Vivo / Beilin Zhang, Brian Kraemer and Dhruba SenGupta [et al.] -- 19. Split Ubiquitin: a Sensor of Protein Interactions In Vivo / Nils Johnsson and Alexander Varshavsky.

ISBN

0195109384

LCCN

96039040

OCLC

ocm35657870

Owning Institutions

Columbia University Libraries