Research Catalog

Recombinant DNA principles and methodologies

Title
Recombinant DNA principles and methodologies / edited by James J. Greene, Venigalla B. Rao.
Publication
New York : Marcel Dekker, [1998], ©1998.

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Additional Authors
  • Greene, James J. (James Joseph)
  • Rao, Venigalla B.
Description
ix, 750 pages : illustrations; 24 cm
Subjects
Bibliography (note)
  • Includes bibliographical references and index.
Contents
  • 1. Why Cloning? / James J. Greene -- 2. Biochemistry of Nucleic Acids / Paul S. Miller -- 3. Enzymology of Recombinant DNA / Frank J. Castora -- 4. Basic Escherichia coli Genetics Useful for Recombinant DNA Techniques / Darren D. Sledjeski and Susan Garges -- 5. Formulation of Cloning Strategies / John B. Dame -- 6. Isolation and Purification of Nucleic Acids / James J. Greene -- 7. Gel Electrophoresis, Southern and Northern Analysis / James J. Greene and Frank J. Castora -- 8. Hybridization Probes / Frank J. Castora and James J. Greene -- 9. cDNA Cloning and the Construction of Recombinant DNA / Dante S. Zarlenga -- 10. Molecular Cloning by Plasmid Vectors / Paulina Balbas and Francisco Bolivar -- 11. Phage Lambda: Basic Biology and Use as a Vector / Susan Frackman and Michael Feiss -- 12. Cosmid Cloning / John Collins and Werner Lindenmaier -- 13. Multipurpose Cloning Vectors / August J. Sick, Joseph M. Fernandez and Jay M. Short --
  • 14. Mammalian Expression Vectors / Ricky R. Hirschhorn and Nava Sarver -- 15. Approaches to the Expression of Foreign Genes / Allan R. Shatzman -- 16. Screening for Specific Recombinant Clones / Lucia Irene Gonzalez-Villasenor and Mark M. Manak -- 17. Principles and Applications of the Polymerase Chain Reaction / Gabriela S. Dveksler and Carl W. Dieffenbach -- 18. DNA Sequencing / Maurice A. Kashdan, Phillip T. Moen, Jr. and Venigalla B. Rao [et al.] -- 19. Site-Directed Mutagenesis / Alfred C. Johnson and Marvin Reitz.
ISBN
0824799895 (alk. paper)
LCCN
98004225
OCLC
ocm38965991
Owning Institutions
Columbia University Libraries