Research Catalog

Gene expression systems : using nature for the art of expression

Title
Gene expression systems : using nature for the art of expression / edited by Joseph M. Fernandez and James P. Hoeffler.
Publication
San Diego : Academic Press, 1999.

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Additional Authors
  • Fernandez, Joseph M.
  • Hoeffler, James P.
Description
xii, 480 pages : illustrations; 24 cm
Summary
  • Recombinant gene expression is the fastest growing area in the study of molecular biology. By the time the Human Genome Project is completed (about 2002), several thousand sequences will be known, but the purpose of the resultant expression products will remain a mystery. Gene discovery requires efficient expression systems for determining the structure and function of gene products.
  • Gene Expression Systems covers a variety of promoters and host organisms that researchers can tailor to their specific needs.
Subject
  • Gene expression
  • Prokaryotes > Genetics
  • Insects > Genetics
  • Transgenic animals > Genetics
  • Eukaryotic cells
  • Gene Expression
  • Prokaryotic Cells
  • Animals, Genetically Modified > genetics
  • Eukaryotic Cells
Bibliography (note)
  • Includes bibliographical references and index.
Contents
  • Introduction / Joseph M. Fernandez and James P. Hoeffler -- The Art of Expression -- Selecting a Suitable Expression System: Considerations -- Genomics and the Future of Protein Expression Systems -- Sect. I. Prokaryotic Expression Systems. 1. Gene Expression Systems Based on Bacteriophage T7 RNA Polymerase / Russell Durbin. 2. Expression Vectors Employing the trc Promoter / Jurgen Brosius. 3. Bacillus Expression: A Gram-Positive Model / Eugenio Ferrari and Brian Miller. 4. araB Expression System in Escherichia coli / Marc Better -- Sect. II. Eukaryotic Expression Systems. 5. Adenoviral Vectors for Protein Expression / Dan J. Von Seggern and Glen R. Nemerow. 6. Expression in the Methylotrophic Yeast Pichia pastoris / James M. Cregg. 7. Recombinant Protein Expression in Pichia methanolica / Christopher K. Raymond. 8. Cytomegalovirus Promoter for Expression in Mammalian Cells / Mark F. Stinski. 9. Inducible Mammalian Expression Systems / Marijane Russell.
  • 10. Protein Expression in Mammalian Cells Using Sindbis Virus / Robert P. Bennett -- Sect. III. Expression in Insect Systems. 11. Drosophila S2 System for Heterologous Gene Expression / Robert B. Kirkpatrick and Allan Shatzman. 12. Baculovirus Expression Vector System / Michael Galleno and August J. Sick -- Sect. IV. Transgenic Expression. 13. Recombinant Protein Expression in Transgenic Mice / Rula Abbud and John H. Nilson. 14. Expression of Recombinant Proteins in the Milk of Transgenic Animals / H. M. Meade, Y. Echelard and C. A. Ziomek [et al.]. 15. Recombinant Protein Expression in Plants / Andreas E. Voloudakis, Yanhai Yin and Roger N. Beachy.
ISBN
0122538404
LCCN
98085621
OCLC
ocm40462599
Owning Institutions
Columbia University Libraries