Research Catalog

Title

Molecular mycobacteriology : [techniques and clinical applications] / edited by Robert-A. Ollar, Nancy D. Connell.

Publication

New York : Marcel Dekker, [1999], ©1999.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Ollar, Robert-A., 1948-
• Connell, Nancy D., 1952-

Description

x, 353 pages : illustrations; 24 cm

Summary

• Molecular Mycobacteriology thoroughly examines techniques such as RNA isolation from mycobacteria ... in situ hybridization with nocardial cells ... paraffin slide culture technology for isolating and identifying Mycobacterium avium complex organisms ... safe handling of organisms in genetic analysis ...
• DNA fingerprinting, or restriction fragment length polymorphism (RFLP) analysis ... element-polymerase chain reaction methods for subtyping strains of Mycobacterium tuberculosis ... pulsed field gel electrophoresis ... mycobacterial shuttle vectors ... and gene expression and recombination of mycobacterium ... and more.
• Written by over 20 world-renowned contributors, Molecular Mycobacteriology is sure to be a standard reference work for years to come for mycobacteriologists, general and clinical microbiologists, molecular biologists, infectious disease specialists, biochemists, pathologists, and upper-level undergraduate and graduate students in these disciplines.

Subjects

• Nocardia
• Molecular microbiology
• Bacteriological Techniques
• Genetic Techniques
• Mycobacterium
• Nocardia > Research > Methodology
• Laboratory Manual
• Mycobacteria > Research > Methodology

Genre/Form

Laboratory Manual.

Bibliography (note)

• Includes bibliographical references and index.

Contents

• 1. Diseases Resulting from Mycobacterial and Nocardial Pathogens / Sheldon T. Brown, Robert-A. Ollar and Donald Armstrong -- 2. Conventional and Molecular Methodologies Applied to Handling and Isolation of Mycobacteria Cells Derived from Clinical Isolates / Vincent J. LaBombardi -- 3. Application of Paraffin Slide Culture Technology for Isolation, Speciation, Antibiotic Sensitivity Testing and DNA extraction for Mycobacterium avium Complex Organisms, Isolation and DNA Extraction for MOTT Mycobacteria and Nocardioform Organisms / Robert-A. Ollar -- 4. The Mycobacterial and Nocardial Genomes and Their Isolation and Manipulation / Nancy D. Connell and Robert-A. Ollar -- 5. Mycobacterial Transposable Elements, Insertion Sequences, and Repetitive DNA / Johnjoe McFadden -- 6. DNA Fingerprinting: RFLP Analysis / Mitchell A. Yakrus -- 7. Double Repetitive Element Polymerase Chain Reaction Method for Subtyping Mycobacterium tuberculosis Strains / Lee W. Riley and Eva H. Harris --
• 8. The Application of Pulsed Field Gel Electrophoresis to Strain Typing of Mycobacteria / Robert D. Arbeit -- 9. Isolation and Characterization of Mycobacterial Plasmids / Marjorie L. Beggs and Tobin J. Hellyer -- 10. Mycobacterial Shuttle Vectors: Homologous and Heterologous Gene Expression / Namcy D. Connell, Ruth A. McAdam and Nathalie Winter -- 11. Mycobacterial Genetics: Gene Expression and Recombination / Jeremy W. Dale and Elizabeth Norman -- 12. Nocardia "In Situ" Hybridization / Gail D. Chapman and Blaine L. Beaman -- 13. RNA Extraction / Philip D. Butcher, Kath M. Sole and Joseph A. Mangan.

ISBN

0824702409 (alk. paper)

LCCN

98050992

OCLC

ocm40347491

Owning Institutions

Columbia University Libraries