Research Catalog

Title

PCR applications : protocols for functional genomics / edited by Michael A. Innis, David H. Gelfand, John J. Sninsky.

Publication

San Diego : Academic Press, [1999], ©1999.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Sninsky, John J.
• Innis, Michael A.
• Gelfand, David H.

Description

xviii, 566 pages, 3 unnumbered pages of plates : illustrations (some color); 24 cm

Subject

• Polymerase chain reaction
• Gene amplification
• Polymerase chain reaction > Laboratory manuals
• Gene amplification > Laboratory manuals
• Polymerase Chain Reaction > methods
• Genetic Engineering
• Genomes
• Polymerase chain reaction > Diagnostic use

Genre/Form

Laboratory Manual.

Bibliography (note)

• Includes bibliographical references and index.

Contents

• Pt. 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- Ch. 3. Thermostable DNA polymerases: an update-- Ch. 4. Musings on microbial genomes-- Ch. 5. Statistical refinement of primer design parameters-- Ch. 6. Multiplex PCR: optimization guidelines-- Ch. 7. The use of immobilized mismatch binding protein for the optimization of PCR fidelity
• Ch. 8. A new generation of PCR instruments and nucleic acid concentration systems-- Ch. 9. Sequencing PCR products-- Ch. 10. Recent advances in high-temperature reverse transcription and PCR-- Ch. 11. Viral genotyping by a quantitative point mutation assay: application to HIV-1 drug resistance-- Ch. 12. In situ PCR-- Pt. 2. Quantitative PCR-- Ch. 13. Standards for PCR assays-- Ch. 14. Rapid thermal cycling and PCR kinetics-- Ch. 15. Kinetics of competitive reverse transcriptase-PCR
• Ch. 16. Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes-- Ch. 17. Quantification of telomerase activity using telomeric repeat amplification protocol-- Pt. 3. Gene discovery-- Ch. 18. Differential display-- Ch. 19. Single-cell cDNA libraries-- Ch. 20. Whole cell assays-- Ch. 21. Screening differentially displayed PCR products by single-strand conformation polymorphism gels-- Ch. 22. Microsatellite protocols-- Ch. 23. Real-time quantitative PCR: uses in discovery research
• Ch. 24. Homology cloning: a molecular taxonomy of the archaea-- Ch. 25. Cloning mammalian homologs of drosophila genes-- Ch. 26. Cloning human homologs of yeast genes-- Pt. 4. Genomics and expression profiling-- Ch. 27. Cellular transcriptome analysis using a kinetic PCR assay-- Ch. 28. Parallel analysis with biological chips-- Ch. 29. High-density cDNA grids for hybridization fingerprinting experiments-- Ch. 30. Comparative genomics hybridization-- Ch. 31. Genetic footprinting and functional maps of the yeast genome
• Ch. 32. Molecular analysis of microdissected tissue: laser capture microdissection-- Ch. 33. Amplified fragmant length polymorphism: studies on plant development-- Ch. 34. A florescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes-- Ch. 35. The Cleavase I enzyme for mutation and polymorphism scanning.

ISBN

• 0123721857
• 0123721865 (pbk)

LCCN

99211203

OCLC

ocm41339460

Owning Institutions

Columbia University Libraries