Research Catalog

Title

Transcriptional regulation in eukaryotes : concepts, strategies, and techniques / Michael Carey, Stephen T. Smale.

Author

Carey, Michael (Michael F.)

Publication

Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press, [2000], ©2000.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Smale, Stephen T.

Description

xxix, 640 pages : illustrations; 29 cm

Summary

• "In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need.
• Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene or the biochemistry of a new transcription factor." "This book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states."--BOOK JACKET.

Subjects

• Genetic transcription > Regulation
• Transcription factors
• Transcription, Genetic
• Transcription Factors
• Genetic transcription > Regulation > Research > Methodology

Bibliography (note)

• Includes bibliographical references and index.

Contents

• 1. A Primer on Transcriptional Regulation in Mammalian Cells. Concepts and Strategies: I. Promoters and the General Transcription Machinery. Concepts and Strategies: II. Activators and Repressors. Concepts and Strategies: III. Chromatin and Gene Regulation. Concepts and Strategies: IV. The Enhanceosome -- 2. Initial Strategic Issues. Concepts and Strategies -- 3. Modes of Regulating mRNA Abundance. Concepts and Strategies. Techniques -- 4. Transcription Initiation Site Mapping. Concepts and Strategies. Techniques -- 5. Functional Assays for Promoter Analysis. Concepts and Strategies. Techniques -- 6. Identification and Analysis of Distant Control Regions. Concepts and Strategies -- 7. Identifying cis-Acting DNA Elements within a Control Region. Concepts and Strategies -- 8. Identification of DNA-Binding Proteins and Isolation of their Genes. Concepts and Strategies for the Identification of DNA-Binding Proteins.
• Concepts and Strategies for Cloning Genes Encoding DNA-Binding Proteins -- 9. Confirming the Functional Importance of a Protein-DNA Interaction. Concepts and Strategies -- 10. In Vivo Analysis of an Endogenous Control Region. Concepts and Strategies. Techniques -- 11. Approaches for the Synthesis of Recombinant Transcription Factors. Concepts and Strategies -- 12. Identifying and Characterizing Transcription Factor Domains. Concepts and Strategies: Defining Domains. Concepts and Strategies: Protein-Protein Interactions. Techniques -- 13. Theory, Characterization, and Modeling of DNA Binding by Regulatory Transcription Factors. Concepts and Strategies. Techniques -- 14. Crude and Fractionated Systems for In Vitro Transcription. Concepts and Strategies. Techniques -- 15. Approaches for Studying Transcription Complex Assembly. Concepts and Strategies. Techniques. App. I. Cautions -- App. II. Suppliers -- App. III. Trademarks.

ISBN

0879695374 (alk. paper)

LCCN

99049636

OCLC

• 42603257
• ocm42603257
• SCSB-3828184

Owning Institutions

Columbia University Libraries