Research Catalog

Title

Animal cell electroporation and electrofusion protocols / edited by Jac A. Nickoloff.

Publication

Totowa, N.J. : Humana Press, 1995.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Nickoloff, Jac A.

Description

xx, 369 pages : illustrations; 23 cm.

Summary

• Animal Cell Electroporation and Electrofusion Protocols provides well-tested protocols for the electroporation of proteins and DNA into insect, fish, and mammalian cells. The collection is distinguished by its coverage of important model cell types from many organisms and tissue types, including Chinese hamster ovary cells, normal human fibroblasts, and human lymphoblastoid cells.
• It also includes detailed instructions for the growth and preparation of specific cells to achieve optimum animal cell transfection and proven electrofusion techniques for studies of somatic cell genetics and of development, as well as for the generation of monoclonal antibodies.
• Animal Cell Electroporation and Electrofusion Protocols is an indispensable guide to animal cell electroporation for graduate and postdoctoral students, as well as laboratory directors in basic, applied, biomedical, biotechnological, and clinical research settings. Its extensive reference lists, citations of alternative transfer methods, advice on pitfalls to avoid, and descriptions of expected results ensure readily reproducible success.

Series Statement

Methods in molecular biology ; 48

Uniform Title

Methods in molecular biology (Clifton, N.J.) ; v. 48.

Subject

• Electroporation
• Electrofusion
• Animal cell biotechnology

Note

• Includes bibliographical references and index.

Contents

• Pt. I. Theory and Instrumentation. Ch. 1. Electroporation Theory: Concepts and Mechanisms / James C. Weaver. Ch. 2. Effects of Pulse Length and Strength on Electroporation Efficiency / Sek Wen Hui. Ch. 3. Instrumentation / Gunter A. Hofmann -- Pt. II. Electroporation Protocols. Ch. 4. The Introduction of Proteins into Mammalian Cells by Electroporation / William F. Morgan and Joseph P. Day. Ch. 5. Electroporation of Antigen-Presenting Cells for T-Cell Recognition and Cytotoxic T-Lymphocyte Priming / Weisan Chen and James McCluskey. Ch. 6. Electroporation of Antibodies into Mammalian Cells / Paul L. Campbell, James McCluskey, Jing Ping Yeo and Ban-Hock Toh. Ch. 7. Electroporation of Adherent Cells In Situ for the Introduction of Nonpermeant Molecules / Leda H. Raptis, Kevin L. Firth, Heather L. Brownell, Andrea Todd, W. Craig Simon, Brian M. Bennett, Leslie W. MacKenzie and Maria Zannis-Hadjopoulos.
• Ch. 8. Electrotransformation of Chinese Hamster Ovary Cells / Danielle Gioioso Taghian and Jac A. Nickoloff. Ch. 9. Electroporation of Rat Pituitary Cells / Ruth H. Paulssen, Eyvind J. Paulssen and Kaare M. Gautvik. Ch. 10. Electroporation of Plasmid DNA into Normal Human Fibroblasts / F. Andrew Ray. Ch. 11. Electroporation-Mediated Gene Transfer into Hepatocytes / Alphonse Le Cam. Ch. 12. Electroporation of Human Lymphoblastoid Cells / Fen Xia and Howard L. Liber. Ch. 13. The Use of Electroporated Bovine Spermatozoa to Transfer Foreign DNA into Oocytes / Marc Gagne, Francois Pothier and Marc-Andre Sirard. Ch. 14. Electroporation of Embryonic Stem Cells for Generating Transgenic Mice and Studying In Vitro Differentiation / John S. Mudgett and Thomas J. Livelli. Ch. 15. Electrotransfection with "Intracellular" Buffer / Maurice J. B. van den Hoff, Vincent M. Christoffels, Wil T. Labruyere, Antoon F. M. Moorman and Wouter H. Lamers.
• Ch. 16. Effect of Cis-Located Human Satellite DNA on Electroporation Efficiency / Djenann Saint-Dic and Michael S. DuBow. Ch. 17. Quantitation of Transient Gene Expression / Michael K. Showe and Louise C. Showe. Ch. 18. Stable Integration of Vectors at High Copy Number for High-Level Expression in Animal Cells / James Barsoum. Ch. 19. Electroporation of Drosophila Embryos / K. Puloma Kamdar, Thao N. Wagner and Victoria Finnerty. Ch. 20. Transformation of Fish Cells and Embryos / Koji Inoue, Jun-ichiro Hata and Shinya Yamashita. Ch. 21. Electroporation of Cardiac Cells / Leslie Tung. Ch. 22. Electroporation for Gene Therapy / Kathryn E. Matthews, Sukhendu B. Dev, Frances Toneguzzo and Armand Keating -- Pt. III. Electrofusion Protocols. Ch. 23. Electrofusion of Mammalian Cells / Kenneth L. White. Ch. 24. Stabilizing Antibody Secretion of Human Epstein Barr Virus-Activated B-Lymphocytes with Hybridoma Formation by Electrofusion / Susan Perkins and Steven K. H. Foung.
• Ch. 25. Electrofusion of Mammalian Oocytes and Embryonic Cells / Josef Fulka, Jr., Robert M. Moor and Josef Fulka. Ch. 26. Nuclear Transfer in Bovine Embryos / Akira Iritani and Tasuku Mitani. Ch. 27. Electrofusion of Mouse Embryos to Produce Tetraploids / Ulrich Petzoldt. Ch. 28. Spectrofluorometric Assay for Cell-Tissue Electrofusion / Richard Heller. Ch. 29. Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products / Mark J. Jaroszeski, Richard Gilbert and Richard Heller.

ISBN

089603304X (alk. paper)

LCCN

95030825

OCLC

• 32739365
• ocm32739365
• SCSB-3862622

Owning Institutions

Columbia University Libraries