Research Catalog

Culture of animal cells : a manual of basic technique

Title
Culture of animal cells : a manual of basic technique / R. Ian Freshney.
Author
Freshney, R. Ian.
Publication
New York : J. Wiley, [2000], ©2000.

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Description
xxvi, 577 pages : illustrations (some color); 29 cm
Summary
  • "The Fourth Edition of Culture of Animal Cells: A Manual of Basic Technique offers the most complete training manual of its kind on the fundamental principles and techniques of animal cell culture. Within this volume, indispensable updates reflecting the latest progress in media, specialized techniques, biotechnology, DNA transfer, and tumor culture have been made.
  • This edition has five new chapters expanding on serum-free media, scale-up and biofermentors, molecular techniques, immortalization, and troubleshooting." "Biomedical researchers in cell biology, cytology, molecular biology, immunology, neuroscience, toxicology, and cancer biology will find Culture of Animal Cells: A Manual of Basic Technique, Fourth Edition to be an invaluable reference."--BOOK JACKET.
Subject
  • Tissue culture > Laboratory manuals
  • Cell culture > Laboratory manuals
  • Cell Culture Techniques
  • Tissue Culture Techniques
  • Tissue culture
Genre/Form
Laboratory Manual.
Bibliography (note)
  • Includes bibliographical references (p. 523-561) and index.
Contents
1. Introduction -- 2. Biology of Cultured Cells -- 3. Design and Layout -- 4. Equipment -- 5. Aseptic Technique -- 6. Safety -- 7. Culture Vessels -- 8. Media -- 9. Serum-Free Media -- 10. Preparation and Sterilization -- 11. Primary Culture -- 12. Cell Lines -- 13. Cloning and Selection -- 14. Cell Separation -- 15. Characterization -- 16. Differentiation -- 17. Transformation -- 18. Contamination -- 19. Cryopreservation -- 20. Quantitation -- 21. Cytotoxicity -- 22. Specialized Cells -- 23. Tumor Cells -- 24. Organotypic Culture -- 25. Scale-Up -- 26. Specialized Techniques -- 27. Molecular Techniques -- 28. Problem Solving.
ISBN
0471348899 (alk. paper)
LCCN
99023536
OCLC
  • ocm41266026
  • SCSB-3863726
Owning Institutions
Columbia University Libraries