Research Catalog

Title

Steroid receptor methods : protocols and assays / edited by Benjamin A. Lieberman.

Publication

Totowa : Humana Press, 2001.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Lieberman, Benjamin A.

Description

xiv, 381 pages : illustrations; 24 cm.

Series Statement

Methods in molecular biology ; 176

Uniform Title

Methods in molecular biology ; v. 176.

Subjects

• Steroid hormones > Receptors > Laboratory manuals
• Laboratory Manual
• Receptors, Steroid

Genre/Form

Laboratory Manual.

Bibliography (note)

• Includes bibliographical references and index.

Contents

• Pt. I. Bioinformatics. 1. Bioinformatics of Nuclear Receptors / Mark Danielsen. 2. Phylogenetic Inference and Parsimony Analysis / Llewellyn D. Densmore III -- Pt. II. Purification Protocols. 3. Expression and Purification of Recombinant Human Progesterone Receptor in Baculovirus and Bacterial Systems / Vida Senkus Melvin and Dean P. Edwards. 4. High-Yield Purification of Functionally Active Glucocorticoid Receptor / Terace M. Fletcher, Barbour S. Warren and Christopher T. Baumann / [et al.]. 5. Production and Purification of Histidine-Tagged Dihydrotestosterone-Bound Full-Length Human Androgen Receptor / Mingmin Liao and Elizabeth M. Wilson. 6. Large Scale Production of Nuclear Receptor Ligand-Binding Domains / Li-Zhi Mi and Fraydoon Rastinejad. 7. Physical Structure of Nuclear Receptor-DNA Complexes / Scott A. Chasse and Fraydoon Rastinejad.
• 8. Isolation of Steroid-Regulated Genes from the Uterus by mRNA Differential Display / Sushma Kumar, Maarit Angervo and Milan K. Bagchi / [et al.]. 9. Identification of Nuclear Hormone Receptor Homologs by Screening Libraries with Highly Degenerate Oligonucleotide Probes / Bruce Blumberg -- Pt. III. Steroid Hormone-Binding Assays. 10. Use of [[superscript 99m]Tc] Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors / Frank Wust. 11. Steroid Hormone Metabolites and Hormone Binding Assays / Rosemary Bland and Martin Hewison. 12. Vitamin D[subscript 3] Analog Screening / Sami Valsanen, Sanna Ryhanen and Pekka H. Maenpaa -- Pt. IV. Protein Interaction Assays. 13. Application of Green Fluorescent Protein to the Study of Dynamic Protein - Protein Interactions and Subcellular Trafficking of Steroid Receptors / Steven K. Nordeen, Paul R. Housley and Yihong Wan / [et al.]. 14. Knockout Mice and Steroid Receptor Research / Per Flodby, Stephan Teglund and Jan-Ake Gustafsson.
• 15. Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors / Bertrand Le Douarin, David M. Heery and Claudine Gaudon / [et al.]. 16. Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex / Rabindra N. Bhattacharjee, Caroline Underhill and Joseph Torchia. 17. Nonradioactive Photoaffinity Labeling of Steroid Receptors Using Western Blot Detection System / Simon J. Evans and Frank L. Moore. 18. Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay / James R. Lambert and Steven K. Nordeen. 19. Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Transcription in Vivo / Christy J. Fryer and Trevor K. Archer. 20. Contransfection Assays and Steroid Receptor Biology / Shimin Zhang and Mark Danielsen. 21. Estrogen Receptor mRNA In Situ Hybridization Using Microprobe System / Hironobu Sasano, Sachiko Matsuzaki and Takashi Suzuki --
• Pt. V. Cancer Research and Drug Discovery. 22. Solid Tumor Cancer Markers and Applications to Steroid Hormone Research / Marcia V. Fournier, Katherine J. Martin and Arthur B. Pardee. 23. Assessing Modulation of Estrogenic Activity of Environmental and Pharmaceutical Compounds Using MCF-7 Focus Assay / Kathleen F. Arcaro and John F. Gierthy. 24. Combinatorial Chemistry in Steroid Receptor Drug Discovery / John A. Flygare, Daniel P. Sutherlin and S. David Brown. 25. Identification of Nuclear Receptor Interacting Proteins Using Yeast Two-Hybrid Technology: Applications to Drug Discovery / Sunil Nagpal, Corine R. Ghosn and Roshantha A. S. Chandraratna.

ISBN

0896037541 (alk. paper)

LCCN

2001024304

OCLC

• ocm46538599
• SCSB-4188860

Owning Institutions

Columbia University Libraries