Research Catalog

Title

Antibody phage display : methods and protocols / edited by Philippa M. O'Brien and Robert Aitken.

Publication

Totowa, N.J. : Humana Press, [2002], ©2002.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• O'Brien, Philippa M.
• Aitken, Robert, 1960-

Description

xiii, 401 pages : illustrations; 23 cm.

Series Statement

Methods in molecular biology ; 178

Uniform Title

Methods in molecular biology (Clifton, N.J.) ; v. 178.

Subjects

• Monoclonal antibodies > Research > Methodology
• Bacteriophages

Bibliography (note)

• Includes bibliographical references and index.

Contents

• 1. Overview of Antibody Phage-Display Technology and Its Applications / Hennie R. Hoogenboom -- 2. Standard Protocols for the Construction of Fab Libraries / Michelle A. Clark -- 3. Standard Protocols for the Construction of scFv Libraries / Simon Lennard -- 4. Broadening the Impact of Antibody Phage Display Technology: Amplification of Immunoglobulin Sequences from Species Other than Humans or Mice / Philippa M. O'Brien and Robert Aitken -- 5. Construction of Large Naive Fab Libraries / Hans J. W. de Haard -- 6. Construction of Polyclonal Antibody Libraries Using Phage Display / Jacqueline Sharon, Seshi R. Sompuram and Chiou-Ying Yang / [et al.] -- 7. Antigen-Driven Stimulation of B-Lymphocytes In Vitro / Zhiwei Hu -- 8. The Recovery of Immunoglobulin Sequences from Single Human B Cells by Clonal Expansion / Ruud M. T. de Wildt and Rene M. A. Hoet -- 9. Panning of Antibody Phage-Display Libraries: Standard Protocols / David W. J. Coomber --
• 10. Selection of Antibodies Against Biotinylated Antigens / Patrick Chames, Hennie R. Hoogenboom and Paula Henderikx -- 11. Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries / Keith A. Charlton and Andrew J. Porter -- 12. Blocking Immunodominant Epitopes by Competitive Deselection / Roberto Burioni -- 13. Rescue of a Broader Range of Antibody Specificities Using an Epitope-Masking Strategy / Henrik J. Ditzel -- 14. Screening of Phage-Expressed Antibody Libraries by Capture Lift / Jeffry D. Watkins -- 15. Antibody-Guided Selection Using Capture-Sandwich ELISA / Kunihiko Itoh and Toshio Suzuki -- 16. Proximity-Guided (ProxiMol) Antibody Selection / Jane K. Osbourn -- 17. Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies / Mariangela Figini and Silvana Canevari -- 18. Selecting Antibodies to Cell-Surface Antigens Using Magnetic Sorting Techniques / Don L. Siegel --
• 19. Isolation of Human Tumor-Associated Cell Surface Antigen-Binding scFvs / Elvyra J. Noronha, Xinhui Wang and Soldano Ferrone -- 20. Subtractive Isolation of Single-Chain Antibodies Using Tissue Fragments / Katarina Radosevic and Willem van Ewijk -- 21. Selection of Antibodies Based on Antibody Kinetic Binding Properties / Ann-Christin Malmborg, Nina Nilsson and Mats Ohlin -- 22. Selection of Functional Antibodies on the Basis of Valency / Manuela Zaccolo -- 23. Two-Step Strategy for Alteration of Immunoglobulin Specificity by In Vitro Mutagenesis / Yoshitaka Iba, Chie Miyazaki and Yoshikazu Kurosawa -- 24. Targeting Random Mutations to Hotspots in Antibody Variable Domains for Affinity Improvement / Partha S. Chowdhury -- 25. Error-Prone Polymerase Chain Reaction for Modification of scFvs / Pierre Martineau -- 26. Use of Escherichia coli Mutator Cells to Mature Antibodies / Robert A. Irving, Gregory Coia and Anna Raicevic / [et al.] --
• 27. Chain Shuffling to Modify Properties of Recombinant Immunoglobulins / Johan Lantto, Pernilla Jirholt and Yvelise Barrios / [et al.] -- 28. Generation of Bispecific and Tandem Diabodies / Sergey M. Kipriyanov -- 29. High-Level Periplasmic Expression and Purification of scFvs / Sergey M. Kipriyanov -- 30. Periplasmic Expression and Purification of Recombinant Fabs Robert L. Raffai -- 31. Expression of Antibody Fragments in Pichia pastoris / Philipp Holliger -- 32. Expression of V[subscript HH] Antibody Fragments in Saccharomyces cerevisiae / J. Marcel van der Vaart -- 33. Intrabodies: Targeting scFv Expression to Eukaryotic Intracellular Compartments / Pascale A. Cohen -- 34. Expression of scFvs and scFv Fusion Proteins in Eukaryotic Cells / Michelle de Graaf, Ida H. van der Meulen-Muileman and Herbert M. Pinedo / [et al.] -- 35. Expression of Antibody Fab Fragments and Whole Immunoglobulin in Mammalian Cells / Pietro P. Sanna.

ISBN

• 0896039064 (hardcover : alk. paper)
• 0896037118

LCCN

2001039568

OCLC

• ocm47894259
• SCSB-4246107

Owning Institutions

Columbia University Libraries