Research Catalog

Title

GTPase protocols : the Ras superfamily / edited by Edward J. Manser and Thomas Leung.

Publication

Totowa, N.J. : Humana Press, [2002], ©2002.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Manser, Ed.
• Leung, Thomas.

Description

xi, 267 pages : illustrations; 24 cm.

Series Statement

Methods in molecular biology ; v. 189

Uniform Title

Methods in molecular biology (Clifton, N.J.) ; v. 189.

Subjects

• Ras proteins > Laboratory manuals
• Guanosine triphosphatase > Laboratory manuals

Bibliography (note)

• Includes bibliographical references and index.

Contents

• Pt. I. General Protocols. 1. The GTPase Cycle: How Dominant Inhibitory Mutants Block the Biological Functions of Small GTPases / Edward J. Manser. 2. Preparation of GTPases for Structural and Biophysical Analysis / Susan J. M. Smith and Katrin Rittinger. 3. Using cDNA-Representational Difference Analysis (cDNA-RDA) in Combination with Microarrays to Identify Rac Regulated Genes / Arndt A. P. Schmitz, Robert Lucito and Linda Van Aelst. 4. Fluorescence Methods in the Study of Small GTP-Binding Proteins / Mohammad Reza Ahmadian, Alfred Wittinghofer and Christian Herrmann -- Pt. II. Specific Methods: Ras Family GTPases. 5. Ras and Rac as Activators of Reactive Oxygen Species (ROS) / Herbert Archer and Dafna Bar-Sagi. 6. Interfering with Ras Signaling Using Membrane-Permeable Peptides or Drugs / Hiroshi Maruta, Hong He and Thao Nheu. 7. A Ras-Based Module to Generate [superscript 32]P-Labeled Fusion Proteins for Blot Overlays / Zhoushen Zhao and Louis Lim --
• Pt. II. Specific Methods: Rho Family GTPases. 8. Determination of the Activity of Rho-Like GTPases in Cells / Angeliki Malliri, Jean P. ten Klooster and Christina Olivio / [et al.]. 9. Introduction of Dominant Inhibitory Proteins Directed Against ROK and MRCK Kinases / Ivan Tan, Louis Lim and Thomas Leung. 10. Effects of Rho Family GTPases on Cell-Cell Adhesion / Masaki Fukata, Masato Nakagawa and Shinya Kuroda / [et al.]. 11. Cell Motility and Invasion Assays / Jackie Banyard and Marc Symons -- Pt. II. Specific Methods: Rab Family GTPases. 12. Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases / Hiroyuki Nakanishi, Takuya Sasaki and Yoshimi Takai. 13. Analysis and Preparation of Stable Complexes between Rab GTPases, Rab Escort Protein, and Rab Geranylgeranyl Transferase / Jose B. Pereira-Leal, Anita Q. Gomes and Miguel C. Seabra -- Pt. II. Specific Methods: Arf Family GTPases.
• 14. Preparation of Myristoylated Arf1 and Arf6 Proteins / Paul A. Randazzo and Henry M. Fales. 15. ARF-Directed Guanine-Nucleotide-Exchange (GEP) Proteins / Gustavo Pacheco-Rodriguez, Joel Moss and Martha Vaughan. 16. Arf6 and its Role of Cytoskeletal Modulation / Julie G. Donaldson. 17. ARF GTPase-Activating Protein 1 / Irit Huber, Edna Cukierman and Miriam Rotman / [et al.] -- Pt. II. Specific Methods: Ran GTPases. 18. The Use of Permeabilized Cell Systems to Study Nuclear Transport / Amy M. Brownawell, James M. Holaska and Ian G. Macara / [et al.]. 19. Analysis of Nuclear Protein Import and Export In Vitro Using Fluorescent Cargoes / Ralph H. Kehlenbach and Larry Gerace. 20. Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton / Sofia B. Lizarraga, Yixian Zheng and Andrew R. Wilde.

ISBN

089603934X (alk. paper)

LCCN

2002022973

OCLC

• ocm49285457
• SCSB-4286728

Owning Institutions

Columbia University Libraries