Research Catalog

Title

New protein techniques / edited by John M. Walker.

Publication

Clifton, N.J. : Humana Press, [1988], ©1988.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Walker, John M., 1948-

Description

xv, 531 pages : illustrations; 24 cm.

Series Statement

• Biological methods
• Methods in molecular biology ; v. 3

Uniform Title

• Biological methods.
• Methods in molecular biology (Clifton, N.J.) ; v. 3.

Subjects

• Proteins
• Proteins > Research > Methodology
• Molecular biology > Research > Methodology
• Molecular Biology > methods
• Eiwitsynthese

Bibliography (note)

• Includes bibliographical references and index.

Contents

Prevention of unwanted proteolysis / Robert J. Beynon -- The Bradford method for protein quantitation / John B.W. Hammond, Nicholas J. Kruger -- Amino acid analysis by precolumn derivatization ; Identification of N-terminal amino acids by high-performance liquid chromatography / E.L.V. Harris -- Enzymatic methods for cleaving proteins ; Chemical cleavage of proteins / Bryan John Smith -- Chemical modification of proteins / Alex F. Carne -- The design, preparation, and use of immunopurification reagents / Andrew Kenney, Lynne Goulding, Christopher Hill -- Dye-ligand chromatography / Sarojani Angal -- Aminohexyl-Sepharose affinity chromatography : purification of an auxin receptor / R.D.J. Barker, H.M. Bailey -- Purification of DNA-dependent RNA polymerase from eubacteria / N.W. Scott -- Direct immunoprecipitation of protein / Christopher F. Thurston, Lucy F. Henley -- Detection of proteins in polyacrylamide gels using an ultrasensitive silver staining technique / Ketan Patel, David J. Easty, Michael J. Dunn -- Chromatofocusing / Ake Sidén, Paolo Gallo -- Hybrid isoelectric focusing using mixed synthetic-carrier ampholyte-immobilized pH gradient gels ; Two-dimensional electrophoresis using immobilized pH gradients in the first dimension ; Two-dimensional polyacrylamide gel electrophoresis using flat-bed isoelectric focusing in the first dimension / Michael J. Dunn, Ketan Patel -- Preparative aspects of immobilized pH gradients / Pier Giorgio Righetti, Cecilia Gelfi -- Isoelectric focusing under denaturing conditions / Christopher F. Thurston, Lucy F. Henley -- Computer analysis of 2-D electrophoresis gels : image analysis, data base, and graphic aids / H. H-S. Ip -- Two-dimensional (crossed) immunoelectrophoresis / John M. Walker -- Peptide synthesis ; Synthesis of a series of analogous peptides using T-bags / Brian Austen -- Production of antisera to synthetic peptides / W.J. Gullick -- Production of antibodies using protein in gel bands / S.A. Amero, T.C. James, S.C.R. Elgin -- Purification of immunoglobulins using protein A-Sepharose / Nicholas J. Kruger, John B.W. Hammond -- Antibody-enzyme conjugate formation / G.B. Wisdom -- Vacuum blotting : an inexpensive, flexible, qualitative blotting technique ; Blotting with plate electrodes / Marnix Peferoen -- Use of dried milk for immunoblotting / Rosemary Jagus, Jeffrey W. Pollard -- Immunodetection of proteins on "Western" blots using ¹²⁵I-labeled protein A / Nicholas J. Kruger, John B.W. Hammond -- Detection of protein blots using the avidin-biotin system / Michael J. Dunn, Ketan Patel -- Detection of protein blots using enzyme-linked second antibodies or protein A / J.M. Walker, W. Gaastra -- Colloidal gold for the detection of proteins on blots and immunoblots / Alan Jones, Marc Moeremans -- Enzyme immobilization by adsorption ; Enzyme immobilization by entrapment ; Enzyme immobilization by covalent bonding ; Cell immobilization / M.D. Trevan.

ISBN

• 0896031268
• 9780896031265

LCCN

92130040

Owning Institutions

Columbia University Libraries