Research Catalog

Title

New nucleic acid techniques / edited by John M. Walker.

Publication

Clifton, N.J. : Humana Press, [1988], ©1988.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Walker, John M., 1948-

Description

xvi, 560 pages : illustrations; 24 cm.

Series Statement

• Biological methods
• Methods in molecular biology ; v. 4

Uniform Title

• Biological methods.
• Methods in molecular biology (Clifton, N.J.) ; v. 4.

Subject

• Molecular Biology > methods
• Molecular biology > Methodology
• Nucleic Acids > analysis
• Nucleic Acids
• Nucleic acids > Methodology
• Nucleïnezuren
• Nucleotide sequence > Methodology
• Wetenschappelijke technieken

Bibliography (note)

• Includes bibliographical references and index.

Contents

Electrophoresis of RNA denatured with glyoxal or formaldehyde / Christopher F. Thurston, Caroline R. Perry, Jeffrey W. Pollard -- Northern blotting / Jeffrey W. Pollard, Caroline R. Perry, Christopher F. Thurston -- Hybrid-release translation / Adrian Slater -- Hybrid-arrested translation / Keith Dudley -- In vitro translation and analysis of early events in protein synthesis initiation in nonreticulocyte mammalian cells / Jeffrey W. Pollard, Michael J. Clemens -- Use of Elutips to purify DNA / Paul D. Van Helden -- Plasmid preparation on Sephacryl S1000 / Paul D. Van Helden, Eileen G. Hoal -- Electrophoresis of DNA in nondenaturing polyacrylamide gels / Robert McGookin -- Use of ³⁵S nucleotides for DNA sequencing / Paul D. Van Helden -- Direct dideoxy DNA sequencing / Alexander Graham, J. Steven, D. McKechnie -- Computer applications to molecular biology : DNA sequences / Robert Harr, Petter Gustafsson -- Detection of sequence-specific protein-DNA interactions by the DNA-footprinting technique / Mark A. Plumb, Graham H. Goodwin -- Oligonucleotide synthesis using the manual phosphotriester method / Daniel M. O'Callaghan, William J. Donnelly -- Manual oligonucleotide synthesis using the phosphoramidite method / H.A. White -- Purification of synthetic oligonucleotides by preparative gel electrophoresis / Robert McGookin -- Isolation of high molecular weight DNA suitable for the construction of genomic libraries / John Steven, Douglas McKechnie, Alexander Graham -- Construction of mammalian genomic libraries using [lambda] replacement vectors / Alan N. Bateson, Jeffrey W. Pollard -- Cosmid library construction / John D. Haley -- Construction of cDNA libraries in [lambda]gt10 or [lambda]gt11 / Michael M. Burrell -- Immunoscreening of [lambda]gt11 expression libraries using an avidin-biotin detection system / Robert McGookin -- Primed synthesis and direct sequencing in the isolation of cDNA clones using short oligonucleotides / John D. Haley -- Constructing expression cDNA libraries using unphosphorylated adaptors / Keith K. Stanley, Joachim Herz, Harald Haymerle -- Expression screening of cDNA libraries in pEX ; Producing antibodies of predetermined specificity from Escherichia coli hybrid proteins ; Epitope mapping using pEX / Keith K. Stanley -- DNA transfection of mammalian cells using polybrene / William G. Chaney ... [et al.] -- Expression of foreign genes in mammalian cells / Martin J. Page -- Dot-blot hybridization method / Hans E.N. Bergmans, Wim Gaastra -- Detection of unique or low copy number DNA sequences in complex genomes / Domingo Gallardo, Albert Boronat -- Photobiotin-labeled DNA and RNA hybridization probes / James L. McInnes, Anthony J. Forster, Robert H. Symons -- Enzyme-labeled DNA probes / J. Lesley Woodhead ... [et al.] -- Luminescent detection of specific DNA sequences ; Detection of DNA sequences using biotinylated probes / J. Lesley Woodhead, Hermia Figueiredo, Alan D.B. Malcolm -- Crosslinking of single-stranded DNA to resins / J. Lesley Woodhead, Jane A. Langdale, Alan D.B. Malcolm -- Extraction of RNA from plants / Adrian Slater -- Isolation of plant nuclei ; In vitro transcription in plant nuclei / Richard D. Henfrey, Robert J. Slater --

ISBN

• 0896031276
• 9780896031272

LCCN

92130060

Owning Institutions

Columbia University Libraries