Research Catalog

Title

ELISA : theory and practice / edited by John R. Crowther.

Publication

Totowa, N.J. : Humana Press, [1995], ©1995.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Crowther, J. R.

Description

xi, 223 pages : illustrations; 23 cm.

Summary

• ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation.
• ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow you to fully appreciate exactly how and why assays function, as well as to efficiently develop assays suitable to your own research that are both rapid and accurate.

Series Statement

Methods in molecular biology ; v. 42

Uniform Title

Methods in molecular biology (Clifton, N.J.) ; v. 42.

Subject

• Enzyme-Linked Immunosorbent Assay
• Enzyme-linked immunosorbent assay

Bibliography (note)

• Includes bibliographical references and index.

Contents

Ch. 1. Basic Immunology -- Ch. 2. Basic Principles of ELISA -- Ch. 3. Stages in ELISA -- Ch. 4. Theoretical Considerations -- Ch. 5. Direct ELISA: Titration of Antigen and Antibody -- Ch. 6. Indirect ELISA -- Ch. 7. Use of Antibodies on Solid-Phase in Capture ELISA -- Ch. 8. Competitive ELISA -- Ch. 9. Immunochemical Techniques.

ISBN

0896032795 (acid-free paper)

LCCN

95007859

Owning Institutions

Columbia University Libraries