Research Catalog

Plant cell electroporation and electrofusion protocols / edited by Jac A. Nickoloff.

Title
Plant cell electroporation and electrofusion protocols / edited by Jac A. Nickoloff.
Publication
Totowa, N.J. : Humana Press, c1995.

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TextRequest in advance QH506 .M56 1995 v. 55Off-site

Details

Additional Authors
Nickoloff, Jac A.
Description
xiv, 205 p. : ill.; 23 cm.
Series Statement
Methods in molecular biology ; 55
Uniform Title
Methods in molecular biology (Clifton, N.J.) ; v. 55.
Subject
  • Crops > Genetic engineering > Laboratory manuals
  • Electrofusion > Laboratory manuals
  • Electroporation > Laboratory manuals
  • Electroporation
  • Plant genetic engineering > Laboratory manuals
  • Plant genetic transformation > Laboratory manuals
  • Plants, Genetically Modified
Genre/Form
Laboratory Manual.
Bibliography (note)
  • Includes bibliographical references and indexes.
Contents
  • Pt. I. Theory and Instrumentation. Ch. 1. Electroporation Theory: Concepts and Mechanisms / James C. Weaver. Ch. 2. Effects of Pulse Length and Strength on Electroporation Efficiency / Sek Wen Hui. Ch. 3. Instrumentation / Gunter A. Hofmann -- Pt. II. Electroporation Protocols. Ch. 4. Electroporation of Agrobacterium tumefaciens / Amke den Dulk-Ras and Paul J. J. Hooykaas. Ch. 5. Electroporation of DNA into the Unicellular Green Alga Chlamydomonas reinhardtii / Laura R. Keller. Ch. 6. Pollen Electrotransformation in Tobacco / James A. Saunders and Benjamin F. Matthews. Ch. 7. Electroporation of Tobacco Leaf Protoplasts Using Plasmid DNA or Total Genomic DNA / Patrick Gallois, Keith Lindsey and Renee Malone. Ch. 8. Electroporation of Brassica / Frank Siegemund and Klaus Eimert. Ch. 9. Transformation of Maize by Electroporation of Embryos / Carol A. Rhodes, Kathleen A. Marrs and Lynn E. Murry.
  • Ch. 10. Transient Gene Expression Analysis in Electroporated Maize Protoplasts / Kathleen A. Marrs and J. C. Carle Urioste. Ch. 11. Reporter Genes and Transient Assays for Plants / Benjamin F. Matthews, James A. Saunders, Joan S. Gebhardt, Jhy-Jhu Lin and Susan M. Koehler -- Pt. III. Electrofusion Protocols. Ch. 12. Electrofusion of Plant Protoplasts: Selection and Screening for Somatic Hybrids of Nicotiana / Harold N. Trick and George W. Bates. Ch. 13. Protoplast Electrofusion and Regeneration in Potato / Jianping Cheng and James A. Saunders. Ch. 14. Polymer-Supported Electrofusion of Protoplasts: A Novel Method and a Synergistic Effect / Lei Zhang.
ISBN
0896033287 (alk. paper)
LCCN
95000358
Owning Institutions
Columbia University Libraries