Research Catalog

Nucleic acid hybridization / M.L.M. Anderson.

Title
Nucleic acid hybridization / M.L.M. Anderson.
Author
Anderson, M. L. M.
Publication
New York : Bios Scientific Publishers, 1999.

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Details

Description
xv, 240 pages : illustrations; 24 cm
Summary
  • In addition, the book will help students and researchers alike to understand the exact methodologies outlined in research articles and scientific journals. Readership: Postgraduates and researchers in cell and molecular biology, genetics and biochemistry, laboratory technicians and research assistants in private and governmental laboratories.
  • This concise, practical guide describes the theory behind nucleic acid hybridization, how it works and the wide variety of ways that it can be employed successfully in cell and molecular biology research. All of the basic methods used for nucleic acid hybridization are presented and the individual steps are described in detail, which will allow Readers to use the techniques immediately.
Subject
  • Nucleic Acid Hybridization
  • Nucleic acid hybridization
Bibliography (note)
  • Includes bibliographical references and index.
Contents
1. Introduction to nucleic acid hybridization -- 2. Types of hybridization and uses of each method -- 3. Solution hybridization: reassociation of DNA -- 4. Solution hybridization: RNA:DNA hybridization -- 5. Solution hybridization: reaction conditions -- 6. Basic types of filter hybridization -- 7. Hybridization strategy: long probes -- 8. Hybridization strategy: oligonucleotide probes -- 9. Choice of probe -- 10. Basic techniques: binding of nucleic acid to filters -- 11. Basic techniques: labeling of probes -- 12. Basic techniques: prehybridization, hybridization and washing -- 13. Basic techniques: detection of hybrids -- 14. Trouble shooting -- 15. Specific applications of techniques -- 16. Current trends -- App. B. Solution hybridization equations -- App. D. Composition of stock solutions -- App. E. Suppliers.
ISBN
0387915567 (softcover : alk. paper)
LCCN
98045587
Owning Institutions
Columbia University Libraries