Research Catalog

Title

Genomic imprinting : methods and protocols / edited by Andrew Ward.

Publication

Totowa, NJ : Humana Press, 2002.

Items in the Library & Off-site

Details

Additional Authors

Ward, Andrew, 1964-

Description

xii, 377 p. : ill.; 24 cm.

Series Statement

Methods in molecular biology ; v. 181

Uniform Title

Methods in molecular biology (Clifton, N.J.) ; v. 181.

Subjects

• Genomic Imprinting
• Laboratory Manual
• Genomic imprinting > Laboratory manuals

Genre/Form

Laboratory Manual.

Bibliography (note)

• Includes bibliographical references and index.

Contents

1. Generation of Monoparental Embryos for Investigation into Genomic Imprinting / Wendy L. Dean, Gavin Kelsey and Wolf Reik -- 2. Deriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic Imprinting / Jeffrey R. Mann -- 3. Balanced Translocations for the Analysis of Imprinted Regions of the Mouse Genome / Anne C. Ferguson-Smith, Maxine Tevendale and Pantelis Georgiades / [et al.] -- 4. Production of YAC Transgenic Mice by Pronuclear Injection / Justin F.-X. Ainscough, Rosalind M. John and Shella C. Barton -- 5. A Transgenic Approach to Studying Imprinted Genes: Modified BACs and PACs / Rosalind M. John, Justin F.-X. Ainscough and Sheila C. Barton -- 6. Methylation-Sensitive Genome Scanning / Izuho Hatada and Tsunehiro Mukal -- 7. Subtraction-Hybridization Method for the Identification of Imprinted Genes / Fumitoshi Ishino, Yoshini Kuroiwa and Naoki Miyoshi / [et al.] -- 8. Identification of Imprinted Loci by Methylation: Use of Methylation-Sensitive Representational Difference Analysis (Me-RDA) / Rachel J. Smith and Gavin Kelsey -- 9. Ribonuclease Protection / Joanne L. Thorvaldsen and Marisa S. Bartolomel -- 10. Quantitative RT-PCR-Based Analysis of Allele-Specific Gene Expression / Judith Singer-Sam and Chunguang Gao -- 11. Allele-Specific In Situ Hybridization (ASISH) / Rolf Ohlsson, Kristian Svensson and Hengmi Cul / [et al.] -- 12. RNA-FISH to Analyze Allele-Specific Expression / Giovanna Braidotti -- 13. Flow Cytometry and FISH to Investigate Allele-Specific Replication Timing and Homologous Association of Imprinted Chromosomes / Janine LaSalle and Marc Lalande -- 14. Southern Analysis Using Methyl-Sensitive Restriction Enzymes / Tom Moore -- 15. A PCR-Based Method for Studying DNA Methylation / Mira Ariel -- 16. Bisulfite-Based Methylation Analysis of Imprinted Genes / Sabine Engemann, Osman El-Maarri and Petra Hajkova / [et al.] -- 17. Direct Analysis of Chromosome Methylation / Deborah Bourc'his and Evani Viegas-Pequignot -- 18. In Vitro Methylation of Predetermined Regions in Recombinant DNA Constructs / Ilse Van den Wyngaert, Roger L. P. Adams and Stefan U. Kass -- 19. In Vitro Methylation of Specific Regions in Recombinant DNA Constructs by Excision and Religation / Ghislaine Dell, Marika Charalambous and Andrew Ward -- 20. Detection of Methyl-Sensitive DNA-Binding Proteins with Possible Involvement in the Imprinting Phenomenon / Kerstin Otte and Bjorn Rozell -- 21. Probing Chromatin Structure with Nuclease Sensitivity Assays / Richard I. Gregory, Sanjeev Khosia and Robert Fell -- 22. Examining Histone Acetylation at Specific Genomic Regions / Ji-Fan Hu and Andrew R. Hoffman -- 23. Purification of the MeCP2/Histone Deacetylase Complex from Xenopus laevis / Peter L. Jones, Paul A. Wade and Alan P. Wolffe -- 24. Reconstitution of Chromatin in Vitro / Klyoe Ura and Yasufumi Kaneda -- 25. Genomic Imprinting in Plants / Rinke Vinkenoog, Melissa Spleiman and Sally Adams / [et al.].

ISBN

089603741X (alk. paper)

LCCN

2001024456

Owning Institutions

Columbia University Libraries