Research Catalog

Title

Directed evolution library creation : methods and protocols / edited by Frances H. Arnold and George Georgiou.

Publication

Totowa, N.J. : Humana Press, c2003.

Items in the Library & Off-site

Details

Additional Authors

• Arnold, Frances Hamilton
• Georgiou, George

Description

x, 224 p. : ill.; 24 cm.

Series Statement

Methods in molecular biology (Clifton, N.J.) ; v. 231

Uniform Title

Methods in molecular biology (Clifton, N.J.) ; v. 231.

Subjects

• Microbial mutation > Laboratory manuals
• Molecular evolution > Laboratory manuals
• Genetic recombination > Laboratory manuals
• Combinatorial chemistry > Laboratory manuals
• Gene libraries > Laboratory manuals
• Genetic engineering > Laboratory manuals
• Combinatorial Chemistry Techniques
• Gene Library

Bibliography (note)

• Includes bibliographical references and index.

Contents

• 1. Generating Mutant Libraries Using Error-Prone PCR / Patrick C. Cirino, Kimberly M. Mayer and Daisuke Umeno -- 2. Preparing Libraries in Escherichia coli / Alexander V. Tobias -- 3. Preparing Libraries in Saccharomyces cerevisiae / Thomas Bulter and Miguel Alcalde -- 4. Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP) / Kentaro Miyazaki -- 5. Construction of Designed Protein Libraries Using Gene Assembly Mutagenesis / Paul H. Bessette, Marco A. Mena, Annalee W. Nguyen and Patrick S. Daugherty -- 6. Production of Randomly Mutated Plasmid Libraries Using Mutator Strains / Annalee W. Nguyen and Patrick S. Daugherty -- 7. Evolution of Microorganisms Using Mutator Plasmids / Olga Selifonova and Volker Schellenberger -- 8. Random Insertion and Deletion Mutagenesis / Hiroshi Murakami, Takahiro Hohsaka and Masahiko Sisido -- 9. Random Oligonucleotide Mutagenesis / Jessica L. Sneeden and Lawrence A. Loeb --
• 10. Saturation Mutagenesis / Radu Georgescu, Geethani Bandara and Lianhong Sun -- 11. DNA Shuffling / John M. Joern -- 12. Family Shuffling with Single-Stranded DNA / Wenjuan Zha, Tongbo Zhu and Huimin Zhao -- 13. In Vitro DNA Recombination by Random Priming / Olga Esteban, Ryan D. Woodyer and Huimin Zhao -- 14. Staggered Extension Process (StEP) In Vitro Recombination / Anna Marie Aguinaldo and Frances H. Arnold -- 15. RACHITT: Gene Family Shuffling by Random Chimeragenesis on Transient Templates / Wayne M. Coco -- 16. The Creation of ITCHY Hybrid Protein Libraries / Marc Ostermeier and Stefan Lutz -- 17. Preparation of SCRATCHY Hybrid Protein Libraries: Size- and In-Frame Selection of Nucleic Acid Sequences / Stefan Lutz and Marc Ostermeier -- 18. Sequence Homology-Independent Protein Recombination (SHIPREC) / Andrew K. Udit, Jonathan J. Silberg and Volker Sieber --
• 19. Producing Chimeric Genes by CLERY: In Vitro and In Vivo Recombination / Valerie Abecassis, Denis Pompon and Gilles Truan -- 20. Analysis of Shuffled Libraries by Oligonucleotide Probe Hybridization / Peter Meinhold, John M. Joern and Jonathan J. Silberg -- 21. Sequence Mapping of Combinatorial Libraries on Macro- or Microarrays: Experimental Design of DNA-Arrays / Valerie Abecassis, Gilles Truan, Loic Jaffrelo and Denis Pompon -- 22. Sequence Mapping of Combinatorial Libraries on Macro- and Microarrays: Bioinformatic Treatment of Data / Denis Pompon, Gilles Truan and Valerie Abecassis.

ISBN

1588292851 (alk. paper)

LCCN

2003007889

OCLC

• ocm52037993
• SCSB-4799018

Owning Institutions

Columbia University Libraries