Research Catalog

Title

Protein kinase C protocols / edited by Alexandra C. Newton.

Publication

Totowa, N.J. : Humana Press, c2003.

Items in the Library & Off-site

Details

Additional Authors

Newton, Alexandra C.

Description

xvi, 582 p. : ill. (some col.); 24 cm.

Series Statement

Methods in molecular biology ; v. 233

Uniform Title

Methods in molecular biology (Clifton, N.J.) ; v. 233.

Subjects

• Protein Kinases
• Laboratory Manual
• Protein kinase C > Laboratory manuals

Genre/Form

Laboratory Manual.

Bibliography (note)

• Includes bibliographical references and index.

Contents

• 1. The Ins and Outs of Protein Kinase C / Alexandra C. Newton -- 2. Early Studies of Protein Kinase C: A Historical Perspective / Yasutomi Nishizuka and Ushio Kikkawa -- 3. Expression and Purification of Protein Kinase C from Insect Cells / Hideyuki Mukai and Yoshitaka Ono -- 4. Expression and Purification of Protein Kinase C[delta] from Bacteria / Michael Gschwendt and Luise Stempka -- 5. Complexities in Protein Kinase C Activity Assays: An Introduction / Julianne J. Sando -- 6. Enzyme Assays for Protein Kinase C Activity / Julianne J. Sando and Jacquelyn K. Beals -- 7. Subcellular Translocation of Protein Kinase C / Julianne J. Sando, Jacquelyn K. Beals and Isa M. Hussaini -- 8. Measuring the Interaction of Protein Kinase C with Membranes: An Introduction / Alexandra C. Newton -- 9. Fluorescence Imaging of Protein Kinase C Translocation in Living Cells / Naoaki Saito --
• 10. Measuring the Binding of Protein Kinase C to Sucrose-Loaded Vesicles / Jennifer Giorgione and Alexandra C. Newton -- 11. Use of Stopped-Flow Fluorescence Spectroscopy to Measure Rapid Membrane Binding by Protein Kinase C / Eric A. Nalefski and Alexandra C. Newton -- 12. [[superscript 3]H]Phorbol 12, 13-Dibutyrate Binding Assay for Protein Kinase C and Related Proteins / Nancy E. Lewin and Peter M. Blumberg -- 13. Protein Kinase C Phosphorylation: An Introduction / Peter J. Parker -- 14. Pulse-Chase Analysis of Protein Kinase C / Mikiko Takahashi and Yoshitaka Ono -- 15. PDK-1 and Protein Kinase C Phosphorylation / Alex Toker -- 16. In Vitro Autophosphorylation of Protein Kinase C Isozymes / Antonio M. Pepio and Wayne S. Sossin -- 17. Tyrosine Phosphorylation of Protein Kinase C / Toshiyoshi Yamamoto, Emiko Yamauchi, Hisaaki Taniguchi, Hidenori Matsuzaki and Ushio Kikkawa --
• 18. Methods to Study Dephosphorylation of Protein Kinase C In Vivo / Karen England and Martin Rumsby -- 19. Phosphopeptide-Specific Antibodies to Protein Kinase C / Wayne S. Sossin -- 20. Identifying Protein Kinase C Substrates: An Introduction / Alex Toker -- 21. A Chemical Genetic Approach for the Identification of Direct Substrates of Protein Kinases / Kavita Shah and Kevan M. Shokat -- 22. Studying the Optimal Peptide Substrate Motifs of Protein Kinase C Using Oriented Peptide Libraries / Michael B. Yaffe -- 23. Structural Analysis of Protein Kinase C: An Introduction / James H. Hurley -- 24. Bacterial Expression and Purification of C1 and C2 Domains of Protein Kinase C Isoforms / Wonhwa Cho, Michelle Digman, Bharath Ananthanarayanan and Robert V. Stahelin -- 25. Crystallization of the Protein Kinase C[delta] C1B Domain / Gongyi Zhang and James H. Hurley -- 26. Methods for Detecting Binding Proteins: An Introduction / Gerda Endemann and Daria Mochly-Rosen --
• 27. Detection of Protein Kinase-Binding Partners by the Yeast Two-Hybrid Analysis / Betty Y. Chang and Christine A. Cartwright -- 28. Glutathione S-Transferase Pull-Down Assay / Deborah Schechtman, Daria Mochly-Rosen and Dorit Ron -- 29. Overlay Method for Detecting Protein-Protein Interactions / Deborah Schechtman, Christopher Murriel, Rachel Bright and Daria Mochly-Rosen -- 30. An Overlay Assay for Detecting Protein Kinase C-Binding Proteins and Substrates / Susan Jaken -- 31. Functional Proteomic Analysis of the Protein Kinase C Signaling System / Jason M. Pass, Jun Zhang, Thomas M. Vondriska and Peipei Ping -- 32. Pharmacological Probes for Protein Kinase C: An Introduction / Marcelo G. Kazanietz -- 33. Applications of Inhibitors for Protein Kinase C and Their Isoforms / Garry X. Shen, Kerrie J. Way, Judith R. C. Jacobs and George L. King -- 34. Phorbol Esters as Probes for the Study of Protein Kinase C Function / Marcelo G. Kazanietz and Patricia S. Lorenzo --
• 35. Irreversible Inactivation of Protein Kinase C Isozymes by Thiol-Reactive Peptide Substrate Analogs / Catherine A. O'Brian, Nancy E. Ward, Feng Chu and Jubilee R. Stewart -- 36. Genetic Approaches to Studying Protein Kinase C: An Introduction / Robert O. Messing -- 37. Animal Models in the Study of Protein Kinase C Isozymes / Doo-Sup Choi and Robert O. Messing -- 38. Genetic Manipulation of Protein Kinase C In Vivo / Alex Toker -- 39. Yeast as a Host to Screen for Modulators and Regulatory Regions of Mammalian Protein Kinase C Isoforms / Amadeo M. Parissenti and Heimo Riedel -- 40. Protein Kinase C in Disease: Cancer / Alan P. Fields and W. Clay Gustafson -- 41. Characterization of the Role of Protein Kinase C Isozymes in Colon Carcinogenesis Using Transgenic Mouse Models / Alan P. Fields, Nicole R. Murray and W. Clay Gustafson -- 42. Alcohol Addiction / M. Foster Olive and Dorit Ron.

ISBN

1588290689 (alk. paper)

LCCN

2003042346

Owning Institutions

Columbia University Libraries