Research Catalog

Title

Protein misfolding and disease : principles and protocols / edited by Peter Bross, Niels Gregersen.

Publication

Totowa, N.J. : Humana Press, c2003.

Items in the Library & Off-site

Details

Additional Authors

• Bross, Peter.
• Gregersen, Niels.

Description

xv, 318 p. : ill.; 23 cm.

Series Statement

Methods in molecular biology ; 232

Uniform Title

Methods in molecular biology (Clifton, N.J.) ; v. 232.

Subjects

• Proteins > Pathophysiology > Laboratory manuals
• Protein Conformation
• Protein folding > Laboratory manuals
• Laboratory Manual
• Protein Folding

Genre/Form

Laboratory Manual.

Bibliography (note)

• Includes bibliographical references and index.

Contents

1. Protein Misfolding, Aggregation, and Degradation in Disease / Niels Gregersen, Lars Bolund and Peter Bross -- 2. Basic Introduction to In Vivo Protein Folding and Its Defects / Peter Bross and Niels Gregersen -- 3. Cystic Fibrosis: Premature Degradation of Mutant Proteins as a Molecular Disease Mechanism / Marina S. Gelman and Ron R. Kopito -- 4. [alpha]1-Antitrypsin Deficiency: Liver Disease Associated With Retention of a Mutant Secretory Glycoprotein in the Endoplasmic Reticulum / David H. Perlmutter -- 5. Parkinson's Disease: [alpha]-Synuclein and Parkin in Protein Aggregation and the Reversal of Unfolded Protein Stress / Lene Diness Jakobsen and Poul Henning Jensen -- 6. Aberrant Protein Folding as the Molecular Basis of Cancer / Melissa D. Scott and Judith Frydman -- 7. Yeast as a Model System to Investigate Protein Conformational Diseases / Christina M. Coughlan and Jeffrey L. Brodsky -- 8. Expression of Recombinant Proteins: An Introduction / Jane Nohr, Karsten Kristiansen and Anne-M. Krogsdam -- 9. Expression of E. coli Systems / Anne-M. Krogsdam, Karsten Kristiansen and Jane Nohr -- 10. Protein Expression in Yeasts / Jane Nohr, Karsten Kristiansen and Anne-M. Krogsdam -- 11. Site-Directed Mutagenesis / Jane Nohr and Karsten Kristiansen -- 12. Pulse-Chase Labeling Techniques for the Analysis of Protein Maturation and Degradation / Annemieke Jansens and Ineke Braakman -- 13. Detection of Aggregates and Protein Inclusions by Staining of Tissues / James E. Galvin -- 14. Study of Mutant Proteins With Folding Defects in Cultured Patient Cells / Gabriele Dodt and Claudia Walter -- 15. E. coli Expression System for Identifying Folding Mutations of Human Adenosine Deaminase / Ines Santisteban, Francisco X. Arredondo-Vega, Shannon Daniels and Michael S. Hershfield -- 16. Characterization of Overexpressed Mutant Proteins in Mammalian Cells / Thomas Juhl Corydon -- 17. Investigation of Unfolded-Protein Response in Cells Expressing Familial Alzheimer's Disease-Linked Presenilin Variants / Gopal Thinakaran, James W. Bowen, Daisuke Ito, Jae-Yoon Leem, Margaret L. Veselits and Naoyuki Sato -- 18. Huntingtin Fragments Form Aggresome-Like Inclusion Bodies in Mammalian Cells / Annett Boeddrich, Rudi Lurz and Erich E. Wanker -- 19. Application of Chemical Chaperones to the Rescue of Folding Defects / Tip W. Loo and David M. Clarke -- 20. Analysis of Defective Subunit Interactions Using the Two-Hybrid System / Belen Perez, Lourdes R. Desviat and Magdalena Ugarte -- 21. Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in the Cytosol / Lourdes R. Desviat, Belen Perez and Magdalena Ugarte -- 22. Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Microsomes / Alan W. Cuthbert and Will Fuller -- 23. Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Mitochondria / Peter Bross, Vibeke Winter, Christina Bak Pedersen and Niels Gregersen -- 24. Investigation of Folding and Degradation of Mutant Proteins Synthesized in Semipermeabilized Cells / Cornelia M. Wilson and Neil J. Bulleid.

ISBN

1588290654 (hbk. : alk. paper)

LCCN

2002192191

Owning Institutions

Columbia University Libraries