Research Catalog

mRNA processing and metabolism : methods and protocols

Title
mRNA processing and metabolism : methods and protocols / edited by Daniel R. Schoenberg.
Publication
Totowa, N.J. : Humana Press, c2004.

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StatusFormatAccessCall NumberItem Location
TextUse in library QP623.5.M47 M765 2004Off-site

Details

Additional Authors
Schoenberg, Daniel R.
Description
xv, 270 p. : ill.; 23 cm.
Series Statement
Methods in molecular biology ; 257
Uniform Title
Methods in molecular biology (Clifton, N.J.) ; v. 257.
Subjects
Bibliography (note)
  • Includes bibliographical references and index.
Contents
1. Using Chromatin Immunoprecipitation to Map Cotranscriptional mRNA Processing in Saccharomyces cerevisiae / Michael-Christopher Keogh and Stephen Buratowski -- 2. Identifying PhosphoCTD-Associating Proteins / Hemali P. Phatnani and Arno L. Greenleaf -- 3. Imaging Alternative Splicing in Living Cells / Eric J. Wagner, Andrea Baines, Todd Albrecht, Robert M. Brazas and Mariano A. Garcia-Blanco -- 4. Tobramycin Affinity Tag Purification of Spliceosomes / Klaus Hartmuth, Hans-Peter Vornlocher and Reinhard Luhrmann -- 5. Using Single-Strand Conformational Polymorphism Gel Electrophoresis to Analyze Mutually Exclusive Alternative Splicing / Alicia M. Celotto and Brenton R. Graveley -- 6. Methods for the Analysis of Adenosine-to-Inosine Editing in RNA / Zuo Zhang and Gordon G. Carmichael -- 7. Assaying Nuclear Messenger RNA Export in Human Cells / Bryan R. Cullen -- 8. RNA Unwinding Assay for DexD/H-Box RNA Helicases / Stephanie S. Tseng-Rogenski and Tien-Hsien Chang -- 9. Approaches for Monitoring Nuclear Translation / Francisco J. Iborra, Dean A. Jackson and Peter R. Cook -- 10. Immunopurification and Analysis of Protein and RNA Components of mRNP in Mammalian Cells / Fabrice Lejeune and Lynne E. Maquat -- 11. Gene Expression Analysis of Messenger RNP Complexes / Luiz O. F. Penalva, Scott A. Tenenbaum and Jack D. Keene -- 12. Using the [lambda]N Peptide to Tether Proteins to RNAs / Julie Baron-Benhamou, Niels H. Gehring, Andreas K. Kulozik and Matthias W. Hentze -- 13. An Efficient System for Cap- and Poly(A)-Dependent Translation In Vitro / Yuri V. Svitkin and Nahum Sonenberg -- 14. A Poly(A) Tail-Responsive In Vitro System for Cap- or IRES-Driven Translation From HeLA Cells / Christian Thoma, Antje Ostareck-Lederer and Matthias W. Hentze -- 15. Assessing Messenger RNA Decapping in Cellular Extracts / Naomi Bergman, Joseph Milone, Elizabeth J. Bates, Mateusz Opyrchal, Vivian Bellofatto and Jeffrey Wilusz -- 16. Analysis of RNA Exonucleolytic Activities in Cellular Extracts / Devi Mukherjee, David T. Fritz, Walter J. Kilpatrick, Min Gao and Jeffrey Wilusz -- 17. Application of Ligation-Mediated Reverse Transcription Polymerase Chain Reaction to the Identification of In Vivo Endonuclease-Generated Messenger RNA Decay Intermediates / Mark N. Hanson and Daniel R. Schoenberg -- 18. Biochemical Dissection of RNA Silencing in Plants / Guiliang Tang and Phillip D. Zamore -- 19. RNA Interference of mRNA Processing Factors in Drosophila S2 Cells / Alicia M. Celotto and Brenton R. Graveley -- 20. RNA Interference by Short Hairpin RNAs Expressed in Vertebrate Cells / Gregory J. Hannon and Douglas S. Conklin.
ISBN
  • 1588292258 (alk. paper)
  • 1592597505 (e-ISBN)
LCCN
2003027588
OCLC
  • ocm53955373
  • SCSB-4863735
Owning Institutions
Columbia University Libraries