Research Catalog

RNA interference, editing, and modification : methods and protocols / edited by Jonatha M. Gott.

Title
RNA interference, editing, and modification : methods and protocols / edited by Jonatha M. Gott.
Publication
Totowa, N.J. : Humana Press, c2004.

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TextRequest in advance QH506 .M56 v.265Off-site
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Details

Additional Authors
Gott, Jonatha M.
Description
xiii, 435 p. : ill.; 24 cm.
Series Statement
Methods in molecular biology, 1064-3745 ; 265
Uniform Title
Methods in molecular biology (Clifton, N.J.) ; v. 265.
Subjects
Genre/Form
Laboratory Manual.
Bibliography (note)
  • Includes bibliographical references and index.
Contents
1. RNA interference : historical overview and significance / Mary K. Montgomery -- 2. Delivery of double-stranded RNA into Caenorhabditis elegans / Dawn Hull and Lisa Timmons -- 3. Induction and biochemical purification of RNA-induced silencing complex from Drosophila S2 cells / Amy A. Caudy and Gregory J. Hannon -- 4. Analysis of gene function in Trypanosoma brucei using RNA interference / Appolinaire Djikeng, Shuiyuan Shen, Christian Tschudi and Elisabetta Ullu -- 5. Short hairpin activated gene silencing in mammalian cells / Patrick J. Paddison, Amy A. Caudy, Ravi Sachidanandam and Gregory J. Hannon -- 6. Geminivirus vectors for transient gene silencing in plants / Nooduan Muangsan and Dominique Robertson -- 7. Posttranscriptional gene silencing in plants / Susan Varsha Wesley, Chris Helliwell, Ming-Bo Wang and Peter Waterhouse -- 8. Identification of microRNAs and other tiny noncoding RNAs by cDNA cloning / Victor Ambros and Rosalind C. Lee -- 9. A historical perspective on RNA editing : how the peculiar and bizarre became mainstream / Donna J. Koslowsky -- 10. Identification of substrates for adenosine deaminases that act on RNA / Daniel P. Morse -- 11. Purification and assay of recombinant ADAR proteins expressed in the yeast Pichia pastoris or in Escherichia coli / Gillian M. Ring, Mary A. O'Connell and Liam P. Keegan -- 12. Isolation of an mRNA-binding protein involved in C-to-U editing / Carri A. Gerber, Anne Relich and Donna M. Driscoll -- 13. In vitro assays for kinetoplastid U insertion-deletion editing and associated activities / Kenneth Stuart, Reza Salavati, R. P. Igo, Jr., Nancy Lewis Ernst, Setty Palazzo and Bingbing Wang -- 14. Identification and characterization of trypanosome RNA editing complex components / Kenneth Stuart, Aswini K. Panigrahi and Achim Schnaufer -- 15. Chimeric templates and assays used to study physarum cotranscriptional insertional editing in vitro / Elaine M. Byrne -- 16. Methods for analysis of mitochondrial tRNA editing in Acanthamoeba castellanii / Amanda J. Lohan and Michael W. Gray -- 17. In vitro RNA editing systems from higher plant chloroplasts / Tetsuro Hirose, Tetsuya Miyamoto, Junichi Obokata and Masahiro Sugiura -- 18. Studying RNA editing in transgenic chloroplasts of higher plants / Ralph Bock -- 19. Detection and quantification of modified nucleotides in RNA using thin-layer chromatography / Henri Grosjean, Gerard Keith and Louis Droogmans -- 20. Functional characterization of 2[superscript 1]-O-methylation and pseudouridylation guide RNAs / Tamas Kiss and Beata E. Jady -- 21. Experimental RNomics : a global approach to identifying small nuclear RNAs and their targets in different model organisms / Alexander Huttenhofer, Jerome Cavaille and Jean-Pierre Bachellerie.
ISBN
  • 1588292428 (alk. paper)
  • 1592597750 (e-book)
LCCN
2003020827
Owning Institutions
Columbia University Libraries