Research Catalog

B cell protocols / edited by Hua Gu, Klaus Rajewsky.

Title
B cell protocols / edited by Hua Gu, Klaus Rajewsky.
Publication
Totowa, N.J. : Humana Press, c2004.

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TextRequest in advance QR185.8.B15 B2127 2004Off-site
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Details

Additional Authors
  • Gu, Hua, PhD.
  • Rajewsky, K., 1936-
Description
xi, 289 p. : ill. (some col.); 24 cm.
Series Statement
Methods in molecular biology ; v. 271
Uniform Title
Methods in molecular biology (Clifton, N.J.) ; v. 271.
Subjects
Genre/Form
Laboratory Manual.
Bibliography (note)
  • Includes bibliographical references and index.
Contents
1. Characterization of B lymphopoiesis in mouse bone marrow and spleen / Richard R. Hardy and Susan A. Shinton -- 2. Characterization of B-cell maturation in the peripheral immune system / Rita Carsetti -- 3. Identification of B-cell subsets: an exposition of 11-color (Hi-D) FACS methods / James W. Tung, David R. Parks, Wayne A. Moore, Leonard A. Herzenberg and Lenore A. Herzenberg -- 4. Analysis of B-cell life-span and homeostasis / Irmgard Forster -- 5. Differentiation of B lymphocytes from hematopoietic stem cells / Paulo Vieira and Ana Cumano -- 6. Analysis of lymphocyte development and function using the RAG-deficient blastocyst complementation system / Faith M. Young, Carl A. Pinkert and Andrea Bottaro -- 7. Conditional gene mutagenesis in B-lineage cells / Stefano Casola -- 8. Analysis of the germinal center reaction and in vivo long-lived plasma cells / Kai-Michael Toellner, Mahmood Khan and Daniel Man-Yuen Sze -- 9. T-cell-dependent immune responses, germinal center development, and the analysis of V-gene sequences / Claudia Berek -- 10. Introduction of genes into primary murine splenic B cells using retrovirus vectors / Kuo-I. Lin and Kathryn Calame -- 11. Immunoglobulin class switching : in vitro induction and analysis / Sven Kracker and Andreas Radbruch -- 12. In vitro and in vivo assays of B-lymphocyte migration / Chantal Moratz and John H. Kehrl -- 13. Analysis of antigen-specific B-cell memory directly ex vivo / Louise J. McHeyzer-Williams and Michael G. McHeyzer-Williams -- 14. B-cell signal transduction : tyrosine phosphorylation, kinase activity, and calcium mobilization / Tilman Brummer, Winfried Elis, Michael Reth and Michael Huber -- 15. Isolation of lipid rafts From B lymphocytes / Anu Cherukuri, Shiang-Jong Tzeng, Arun Gidwani, Hae Won Sohn, Pavel Tolar, Michelle D. Snyder and Susan K. Pierce -- 16. Molecular single-cell PCR analysis of rearranged immunoglobulin genes as a tool to determine the clonal composition of normal and malignant human B cells / Ralf Kuppers -- 17. Analysis of B-cell immune tolerance induction using transgenic mice / Helen Ferry and Richard J. Cornall -- 18. Analysis of B-cell signaling using DT40 B-cell line / Tomoharu Yasuda and Tadashi Yamamoto -- 19. B-cell development and pre-B-1 cell plasticity in vitro / Antonius G. Rolink.
ISBN
1588293475 (alk. paper)
LCCN
2004002869
Owning Institutions
Columbia University Libraries