Research Catalog
Basic cell culture protocols.
- Title
- Basic cell culture protocols.
- Publication
- Totowa, N.J. : Humana Press, [2005], ©2005.
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1 Item
| Status | Format | Access | Call Number | Item Location |
|---|---|---|---|---|
| Text | Request in advance | QH585.2 .B375 2005 | Off-site |
Holdings
Details
- Additional Authors
- Description
- xii, 371 pages : illustrations; 22 cm +
- Summary
- "Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today."--BOOK JACKET.
- Series Statement
- Methods in molecular biology ; 290
- Uniform Title
- Methods in molecular biology (Clifton, N.J.) ; v. 290.
- Subject
- Genre/Form
- Laboratory Manual.
- Bibliography (note)
- Includes bibliographical references and index.
- System Details (note)
- Minimum system requirements: Adobe reader 6.0 or later.
- Contents
- 1. Culture of primary adherent cells and a continuously growing nonadherent cell line / Cheryl D. Helgason -- 2. Detection of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- 3. Eradication of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- 4. Authentication of scientific human cell lines : easy-to-use DNA fingerprinting / Wilhelm G. Dirks and Hans G. Drexler -- 5. Cytogenetic analysis of cell lines / Roderick A. F. MacLeod and Hans G. Drexler -- 6. Human and mouse hematopoietic colony-forming cell assays / Cindy L. Miller and Becky Lai -- 7. Isolation and culture of murine macrophages / John Q. Davies and Siamon Gordon -- 8. Isolation and culture of human macrophages / John Q. Davies and Siamon Gordon -- 9. Development of T-lymphocytes in mouse fetal thymus organ culture / Tomoo Ueno, Cunlan Liu, Takeshi Nitta and Yousuke Takahama -- 10. In vitro generation of lymphocytes from embryonic stem cells / Renee F. de Pooter, Sarah K. Cho and Juan Carlos Zuniga-Pflucker -- 11. Hematopoietic development of human embryonic stem cells in culture / Xinghui Tian and Dan S. Kaufman -- 12. Generation of murine stromal cell lines : models for the microenvironment of the embryonic mouse aorta-gonads-mesonephros region / Robert A. J. Oostendorp, Kirsty Harvey and Elaine A. Dzierzak -- 13. Culture of human and mouse mesenchymal cells / Emer Clarke -- 14. Isolation, purification, and cultivation of murine and human keratinocytes / Frizell L. Vaughan and Ludmila I. Bernstam -- 15. Isolation and culture of primary human hepatocytes / Edward L. LeCluyse, Eliane Alexandre, Geraldine A. Hamilton, Catherine Viollon-Abadie, D. James Coon, Summer Jolley and Lysiane Richert -- 16. Primary kidney proximal tubule cells / Mary Taub -- 17. Enzymatic dissociation and culture of normal human mammary tissue to detect progenitor activity / John Stingl, Joanne T. Emerman and Connie J. Eaves -- 18. Generation and differentiation of neurospheres from murine embryonic day 14 central nervous system tissue / Sharon A. Louis and Brent A. Reynolds -- 19. Isolation and culture of skeletal muscle myofibers as a means to analyze-satellite cells / Gabi Shefer and Zipora Yablonka-Reuveni -- 20. Adult ventricular cardiomyocytes : isolation and culture / Klaus-Dieter Schluter and Daniela Schreiber -- 21. Isolation and culture of primary endothelial cells / Bruno Larrivee and Aly Karsan -- 22. Studying leukocyte rolling and adhesion in vitro under flow conditions / Susan L. Cuvelier and Kamala D. Patel -- 23. Isolation and characterization of side population cells / Margaret A. Goodell, Shannon McKinney-Freeman and Fernando D. Camargo -- 24. Scalable production of embryonic stem cell-derived cells / Stephen M. Dang and Peter W. Zandstra.
- ISBN
- 1588295451 (pbk. : alk. paper)
- 1588292843 (hardcover : alk. paper)
- 1592598382 (ebook)
- LCCN
- 2004011235
- 9781588292841
- OCLC
- ocm55230476
- SCSB-5103190
- Owning Institutions
- Columbia University Libraries