Research Catalog

Title

Nuclear reprogramming : methods and protocols / edited by Steve Pells.

Publication

Totowa, N.J. : Humana Press, [2006], ©2006.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Pells, Steve.

Description

xiv, 333 pages : illustrations; 24 cm.

Series Statement

Methods in molecular biology ; 325

Uniform Title

Methods in molecular biology (Clifton, N.J.) ; v. 325.

Subjects

• Cell Nucleus > physiology
• Cell nuclei > Transplantation > Laboratory methods
• Transplantation > methods
• Cell Fusion > methods

Bibliography (note)

• Includes bibliographical references and index.

Contents

• Nuclear transplantation in xenopus / J. B. Gurdon -- Nuclear transfer in the sheep / William A. Ritchie -- Protocols for nuclear transfer in mice / Shaorong Gao -- Isolation of stromal stem cells from human adipose tissue / Andrew C. Boquest ... [et al.] -- Nuclear reprogramming by cell fusion / Robert H. Broyles ... [et al.] -- Polyethylene glycol-mediated cell fusion / Jian Yang and Ming Hong Shen -- Epigenetic reprogramming of somatic genomes by electrofusion with embryonic stem cells / Masako Tada and Takashi Tada -- Quantification of cell fusion by flow cytometry / Stephen Sullivan ... [et al.] -- Modulation of cell fate using nuclear and cytoplasmic extracts / Anne-Mari Høkelien, Kristine G. Gaustad, and Philippe Collas -- Transgenic systems in nuclear reprogramming / Megan Munsie, Peter Mountford, and Jennifer Nichols -- Using immunofluorescence to observe methylation changes in mammalian preimplantation embryos / Føtima Santos and Wendy Dean -- Observing nuclear structure/chromatin changes with fluorescently labeled antibodies / Rong Wu, Anna V. Terry, and David M. Gilbert -- Quantitative analysis of telomerase activity and telomere length in domestic animal clones / Dean H. Betts ... [et al.] --
• Pluripotency: capacity for in vitro differentiation of undifferentiated embryonic stem cells / Cornelia Wiese ... [et al.] -- Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens / Bruce A. Fenderson ... [et al.] -- Analysis of the nucleolar compartment of the nucleus as an indicator of nuclear reprogramming after nuclear transfer / Jacques-E. Fløchon -- Methylation-sensitive polymerase chain reaction / Hannah R. Moore, Richard R. Meehan, and Lorraine E. Young -- Analysis of DNA methylation profiles in preimplantation embryos using bisulfite mutagenesis / Yong-Mahn Han, Seok-Ho Kim, and Yong-Kook Kang -- Chromatin immunoprecipitation assay for mammalian tissues / Fiona B. Turner, Wang L. Cheung, and Peter Cheung -- Histone modifications and transcription factor binding on chromatin: CHIP/PCR assays / Jaejoon Won and Tae Kook Kim -- In vivo genomic footprinting using LM/PCR methods / Hiromi Tagoh, Peter N. Cockerill, and Constanze Bonifer -- Analyzing histone modification using crosslinked chromatin treated with micrococcal nuclease / Pascal Lefevre and Constanze Bonifer.

ISBN

1588293793 (alk. paper)

LCCN

2005020464

OCLC

• ocm61121123
• SCSB-5222765

Owning Institutions

Columbia University Libraries