Research Catalog

Title

Techniques for molecular biology / editors, D. Tagu, C. Moussard.

Publication

Enfield, NH : Science Publishers, [2006], ©2006.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Tagu, D. (Denis), 1961-
• Moussard, C. (Christian), 1952-

Description

vii, 227 pages : illustrations; 25 cm

Uniform Title

Principes des techniques de biologie moléculaire. English.

Subject

• Molecular biology > Technique
• Molecular genetics > Technique
• Plant molecular biology > Technique

Note

• Translation of: Principes des techniques de biologie moléculaire. 2e ed. rev. et augm. Paris : INRA, 2003.

Contents

I. Definition -- 1. Structure and expression of a eukaryote gene coding for an mRNA and a protein -- 2. Parameters of description of a genome -- 3. Sequencing of whole genomes -- II. Vectors and cloning -- 4. Restriction enzymes -- 5. Electrophoresis of nucleic acids -- 6. Description of a plasmid and a phagemid -- 7. Description of a bacteriophage and a cosmid -- 8. Description of a YAC and other large vectors -- 9. Molecular cloning -- 10. Genetic transformation of bacteria and yeasts -- III. Labelling of nucleic acids and hybridization -- 11. DNA labelling -- 12. Molecular hybridization -- 13. In situ hybridization of mRNA -- IV. DNA libraries and screening -- 14. Construction of a genomic DNA library -- 15. Construction of a cDNA library -- 16. Screening of a library -- 17. Differential screening : subtractive libraries, AFLP-cDNA -- 18. Differential display RT-PCR -- 19. Differential screening by SSH : suppression subtractive hybridization -- 20. Differential screening by RDA : representational difference analysis -- 21. EST : expressed sequence tags -- 22. DNA microarrays : DNA microchips, cDNA filters -- V. Characterization of a gene -- 23. DNA sequencing -- 24. PCR : polymerase chain reaction -- 25. RACE : rapid amplification of cDNA ends -- 26. Genome walking by PCR -- 27. RT-PCR : reverse transcriptase PCR -- 28. In vitro transcription -- 29. Determination of transcription initiation site -- 30. Functional analysis of promoters -- 31. Gel retardation -- 32. DNase I footprinting -- VI. Genetic transformation of eukaryotes -- 33. Genetic transformation of plants by Agrobacterium tumefaciens -- 34. Direct gene transfer into plant protoplasts -- 35. Direct gene transfer by biolistics -- 36. Genetic transformation of animal cells -- 37. Cloning of animals -- 38. Transient expression -- VII. Analysis of gene function -- 39. Recombinant proteins -- 40. Baculoviruses of insects, vectors of expression of foreign genes -- 41. Yeast two hybrid system -- 42. Site-directed mutagenesis -- 43. Mutation complementation in yeast -- 44. Knock-out of genes in yeast -- 45. Gene tagging -- 46. RNA interference -- VIII. Polymorphism of a genome -- 47. Molecular markers -- 48. Genetic and physical maps -- 49. PFGE : pulse field gel electrophoresis -- 50. RFLP : restriction fragment length polymorphism -- 51. RAPD : random amplified polymorphic DNA -- 52. AFLP : amplified fragment length polymorphism -- 53. Retromarkers -- 54. SSCP : single strand conformation polymorphism -- 55. DGGE : denaturing gel gradient electrophoresis -- 56. SNP : single nucleotide polymorphism -- 57. Simple sequence repeats (SSR) : microsatellites.

ISBN

1578083613

LCCN

• 2005051569
• 9781578083619

OCLC

• ocm61027829
• SCSB-5252701

Owning Institutions

Columbia University Libraries