Research Catalog

Title

Enzyme kinetics : behavior and analysis of rapid equilibrium and steady state enzyme systems / Irwin H. Segel.

Author

Segel, Irwin H., 1935-

Publication

New York : Wiley, 1993.

Items in the Library & Off-site

Holdings

Details

Description

xxii, 957 pages : illustrations; 23 cm

Subjects

• ENZIMAS
• Katalyse
• Enzymen
• Kinetics
• Enzymes > pharmacokinetics
• Enzyme kinetics

Note

• "A Wiley-Interscience publication."

Bibliography (note)

• Includes bibliographies and index.

Contents

Ch. 1. Introduction - Enzymes as Biological Catalysts -- A. The Discovery of Enzymes and the Development of Enzymology -- B. Life, Energy, and Coupled Reactions -- C. Enzymes as Catalysts -- D. The Active Site -- E. Three-Point Attachment -- F. The Flexible Enzyme-Induced Fit Hypothesis -- G. Factors Responsible for the Catalytic Efficiency of Enzymes -- H. Enzyme Kinetics -- Ch. 2. Kinetics of Unireactant Enzymes -- A. The Henri Equation and the Michaelis-Menten Equation -- B. General Rules for Writing Velocity Equations for Rapid Equilibrium Systems -- C. The Van Slyke Equation -- D. The Briggs-Haldane Steady-State Approach -- E. Reversible Reactions - Effect of Product on Forward Velocity -- F. Haldane Relationship Between Kinetic Constants and the Equilibrium Constant -- G. Specific (or Relative or Reduced) Substrate Concentration and Velocity -- H. Velocity Versus Substrate Concentration Curve -- I. Reaction Order -- J. Graphical Determination of K[subscript m] and V[subscript max] -- K. Integrated Form of the Henri-Michaelis-Menten Equation -- L. Multiple Enzymes Catalyzing the Same Reaction -- M. Kinetic Behavior at High Enzyme Concentrations -- N. Enzyme Assays -- O. Effects of Endogenous Substrates -- Ch. 3. Simple Inhibition Systems -- A. Competitive Inhibition (Simple Intersecting Linear Competitive Inhibition) -- B. Noncompetitive Inhibition (Simple Intersecting Linear Noncompetitive Inhibition) -- C. Uncompetitive Inhibition (Simple Linear Uncompetitive Inhibition) -- D. Effects of Contaminating Inhibitors on the Initial Velocity Versus Enzyme Concentration Plot -- E. Tightly Bound Inhibitors -- Ch. 4. Rapid Equilibrium Partial and Mixed-Type Inhibition -- A. Partial Competitive Inhibition (Simple Intersecting Hyperbolic Competitive Inhibition) -- B. Partial Noncompetitive Inhibition (Simple Intersecting Hyperbolic Noncompetitive Inhibition) -- C. Mixed-Type Inhibition -- D. Reciprocal Plot Nomenclature -- E. Interaction Between Inhibitor and Substrate -- F. Other Methods of Plotting Enzyme Kinetics Data -- Ch. 5. Enzyme Activation -- A. Nonessential Activation -- B. Substrate-Activator Complex Is the True Substrate -- Ch. 6. Rapid Equilibrium Bireactant and Terreactant Systems -- A. Random Bireactant Systems -- B. Ordered Bireactant Systems -- C. Random Terreactant Systems -- D. Ordered and Hybrid Random-Ordered Terreactant Systems -- E. Rules for Predicting Inhibition Patterns in Rapid Equilibrium Systems -- Ch. 7. Multisite and Allosteric Enzymes -- A. Enzymes With Multiple Catalytic Sites -- B. Inhibition and Activation in Multisite Systems -- C. The General Sequential Interaction Model of Koshland, Nemethy, and Filmer (Restricted Interactions Between Sites) -- D. The Symmetry Model of Allosteric Enzymes (The Concerted Transition Model of Monod, Wyman, and Changeux) -- E. Alternative Kinetic Explanations for Sigmoidal Responses -- Ch. 8. Multiple Inhibition Analysis -- A. Multiple Sites for a Given Inhibitor -- B. Inhibition by Mixtures of Different Inhibitors -- Ch. 9. Steady-State Kinetics of Multireactant Enzymes -- A. The King-Altman Method of Deriving Steady-State Velocity Equations -- B. Simplification of Complex King-Altman Patterns -- C. General Rules for Defining Kinetic Constants and Deriving Velocity Equations -- D. Iso Uni Uni System (Mobile Carrier Model of Membrane Transport) -- E. Ordered Uni Bi and Ordered Bi Uni Systems -- F. Ordered Bi Bi System -- G. Partial Rapid Equilibrium Ordered Bi Bi System -- H. Theorell-Chance Bi Bi System -- I. Ping Pong Bi Bi System -- J. Partial Rapid Equilibrium Ping Pong Bi Bi Systems -- K. Hybrid Ping Pong - Rapid Equilibrium Random (Two-Site) Bi Bi Systems -- L. Iso Bi Bi Systems -- M. Hybrid Theorell-Chance Ping Pong (and Iso Ping Pong) Systems -- N. Rapid Equilibrium Random Bi Bi Systems -- O. Steady-State Random Mechanisms -- P. Partial Rapid Equilibrium Random Bi Bi System -- Q. Varieties of Nonhyperbolic Velocity Curves -- R. Ordered Ter Bi System -- S. Bi Uni Uni Uni Ping Pong Ter Bi System -- T. Ordered Ter Ter System -- U. Partial Rapid Equilibrium Ordered Terreactant Systems -- V. Ordered Terreactant Systems With Rapid Equilibrium Random Sequences -- W. Bi Uni Uni Bi Ping Pong Ter Ter System -- X. Bi Bi Uni Uni Ping Pong Ter Ter System -- Y. Hexa Uni Ping Pong System -- Z. Summary of Nonrandom Terreactant Systems -- AA. Other Possible Terreactant Systems -- BB. Quadreactant Systems -- CC. General Rules for Predicting Initial Velocity Patterns -- DD. Dead-End Inhibition -- EE. Mixed Dead-End and Product Inhibition -- FF. Inhibition by Alternative Substrates -- GG. Inhibition by Alternative Products -- HH. Substrate Inhibition -- II. A Review of Inhibition Systems -- Ch. 10. Isotope Exchange -- A. Ordered Bi Bi -- B. Random Bi Bi System -- C. Isotope Exchange During A Net Reaction -- D. Ping Pong Systems -- E. Determining Exchange Velocities -- F. Determining K[subscript eq] -- G. Derivation of Isotope Exchange Velocity Equations -- Ch. 11. Effects of pH and Temperature -- A. Effect of pH -- B. Effect of Temperature. Appendix Least Squares Method.

ISBN

0471303097

OCLC

• ocm31157638
• SCSB-5282731

Owning Institutions

Columbia University Libraries