Research Catalog

Title

Advanced bacterial genetics : use of transposons and phage for genomic engineering / edited by Kelly T. Hughes, Stanley R. Maloy.

Publication

Amsterdam ; Boston : Elsevier : Academic Press, [2007], ©2007.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Hughes, Kelly Thomas.
• Maloy, Stanley R.

Description

xxxiv, 284 pages : illustrations; 24 cm.

Series Statement

Methods in enzymology, 0076-6879 ; v. 421

Uniform Title

Methods in enzymology ; v. 421.

Subjects

• Genetics, Microbial
• Transposons
• Bacterial genetics
• Bacteriophages
• DNA Transposable Elements

Bibliography (note)

• Includes bibliographical references and indexes.

Contents

Strain collections and genetic nomenclature / S.R. Maloy ... [et al.] -- Use of antibiotic-resistant transposons for mutagenesis / S.R. Maloy -- In vivo mutagenesis using EZ-Tn5 / J.R. Kirby -- Identification of essential genes in bacteria / D.R. Hillyard ... [et al.] -- Isolation of transposon insertions / S.R. Maloy -- Localized mutagenesis / S.R. Maloy -- Generation of deletions and duplications using transposons as portable regions of homology with emphasis on Mud and Tn10 transposons / K.T. Hughes -- Target-directed proteolysis in vivo / M. Eser ... [et al.] -- Sets of transposon-generated sequence-tagged mutants for structure-function analysis and engineering / B. Traxler ... [et al.] -- Using genomic microarrays to study insertional/transposon mutant libraries / D.N. Baldwin ... [et al.] -- Screening transposon mutant libraries using full-genome oligonucleotide microarrays / K. M. Winterberg ... [et al.] -- Creating recombination-activated genes and sequence-defined mutant libraries using transposons / L. Gallagher ... [et al.] -- Use of operon and gene fusions to study gene regulation in Salmonella / K.T. Hughes ... [et al.] -- Genomic screening for regulatory genes using the T-POP transposon / C. Lee ... [et al.] -- Recombineering: In vivo genetic engineering in E. coli, S. enterica, and beyond / J.A. Sawitzke ... [et al.] -- [lambda]-red genetic engineering in Salmonella enterica serovar Typhimurium / J.E. Karlinsey -- Probing nucleoid structure in bacteria using phage lambda integrase-mediated chromosome rearrangements / N. Garcia-Russell ... [et al.] -- Dissecting nucleic acid-protein interactions using challenge phage / S.R. Maloy ... [et al.] -- Mud-P22 / J.A. Crain ... [et al.] -- Phage metagenomics / V. Casas ... [et al.].

ISBN

• 0123737494
• 9780123737496

OCLC

• ocm85837062
• 85837062
• SCSB-5320665

Owning Institutions

Columbia University Libraries