Research Catalog

Title

Intermediate filament cytoskeleton / edited by M. Bishr Omary, Pierre A. Coulombe.

Publication

Amsterdam ; Boston : Elsevier Academic Press, [2004], ©2004.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Omary, M. Bishr.
• Coulombe, Pierre A.

Description

xxix, 886 pages, 19 unnumbered pages of plates : illustrations (some color).

Summary

"Intermediate filaments, microfilaments and microtubules are the three major cytoskeletal protein families. Several features highlight intermediate filaments as reflected in part by their structure, mode of assembly, diversity, tissue selective expression, and association with a broad range of diseases including skin, liver, neuromuscular, cardiac, eye and hair diseases. Intermediate Filament Cytoskeleton provides a comprehensive resource of methodology essentials for studying intermediate filaments in a broad range of organisms and biological contexts. It offers user-friendly advice and protocols covering all aspects of intermediate filament research including protein isolation and structure, protein and gene regulation, relationship to disease and apoptosis, and associated proteins. Mammalian and non-mammalian systems, and animal models for human diseases are covered, making this book a must-have for any investigator wishing to study IF genes or their protein products. Book jacket."--BOOK JACKET.

Series Statement

Methods in cell biology, 0091-679x ; v. 78

Uniform Title

Methods in cell biology ; v. 78.

Subject

• Intermediate Filament Proteins > physiology
• Cytoskelet
• Filaments cytoplasmiques
• Cytoskeletal proteins
• Cytoplasmic filaments

Genre/Form

Laboratory Manual.

Bibliography (note)

• Includes bibliographical references and index.

Contents

• Part I. General Methods to Study Intermediate Filament Proteins &3- From Molecules to Disease -- 1. Isolation, Characterization, and In Vitro Assembly of Intermediate Filaments / Harald Herrmann, Laurent Kreplak and Ueli Aebi -- I. Introduction -- II. Materials and Methods -- III. Pearls and Pitfalls -- IV. Concluding Remarks -- 2. Intermediate Filament Protein Structure Determination / Sergei V. Strelkov, Laurent Kreplak, Harald Herrmann and Ueli Aebi -- I. Introduction -- II. Approaches and Methods -- III. Pearls and Pitfalls -- IV. Concluding Remarks -- 3. Intracellular Microrheology as a Tool for the Measurement of the Local Mechanical Properties of Live Cells / Thomas P. Kole, Yiider Tseng and Denis Wirtz -- I. Introduction -- II. Materials and Instrumentation -- III. Procedures -- IV. Pearls and Pitfalls -- V. Concluding Remarks -- 4. Developing Mouse Models to Study Intermediate Filament Function / Thomas M. Magin, Michael Hesse, Roland Meier-Bornheim and Julia Reichelt -- I. Introduction -- II. Essential Materials and Methods -- III. Websites, Tables, Reagents, and Vendors of Interest -- 5. Uncovering the Roles of Intermediate Filaments in Apoptosis / Normand Marceau, Stephane Gilbert and Anne Loranger -- I. Introduction -- II. Materials and Instrumentation -- III. Procedures -- IV. Pearls and Pitfalls -- V. Conclusion and Perspectives -- 6. Molecular Genetics Methods for Human Intermediate Filament Diseases / Frances J. D. Smith, Aileen Sandilands and W. H. Irwin McLean -- I. Introduction -- II. Materials and Instrumentation -- III. Procedures -- IV. Pearls and Pitfalls -- V. Discussion and Concluding Remarks -- 7. Intermediate Filaments and Multiparameter Flow Cytometry for the Study of Solid Tumors / Math P. G. Leers -- I. Introduction -- II. Materials and Instrumentation -- III. Procedures -- IV. Comments -- V. Pearls and Pitfalls -- 8. Intermediate Filament Protein Inclusions / Kurt Zatloukal, Conny Stumptner, Andrea Fuchsbichler, Elke Janig and Helmut Denk -- I. Introduction -- II. Materials and Instrumentation -- III. Procedures -- IV. Pitfalls -- V. Discussion -- 9. Stress Models for the Study of Intermediate Filament Function / E. Birgitte Lane and Milos Pekny -- I. Introduction -- II. Materials and Instrumentation -- III. Procedures -- IV. Pitfalls -- V. Discussion/Concluding Remarks -- Part II. General Methods to Study Intermediate Filament Proteins and Gene Regulation -- 10. Regulation of Intermediate Filament Gene Expression / Satrajit Sinha -- I. Introduction -- II. Materials -- III. Procedures -- IV. Pitfalls -- V. Discussion and Concluding Remarks -- 11. Fluorescence-Based Methods for Studying Intermediate Filaments / Eric W. Flitney and Robert D. Goldman -- I. Introduction -- II. Materials and Methods -- III. Pearls and Pitfalls -- IV. Discussion -- 12. Imaging of Keratin Dynamics during the Cell Cycle and in Response to Phosphatase Inhibition / Reinhard Windoffer and Rudolf E. Leube -- I. Introduction -- II. Materials and Instrumentation -- III. Procedures -- IV. Pearls and Pitfalls -- V. Discussion and Concluding Remarks -- 13. Approaches to Study Phosphorylation of Intermediate Filament Proteins Using Site-Specific and Phosphorylation State-Specific Antibodies / Aie Kawajiri and Masaki Inagaki -- I. Introduction -- II. Materials and Instrumentation -- III. Procedures -- IV. Pearls and Pitfalls -- V. Discussion and Concluding Remarks -- 14. Approaches to Study Posttranslational Regulation of Intermediate Filament Proteins / Vitaly Kochin, Hanna-Mari Pallari, Harish Pant and John E. Eriksson -- I. Introduction -- II. Materials and Methods: Characterization of Intermediate Filament Phosphorylation -- III. Pearls and Pitfalls -- IV. Conclusions -- Part III. Methods to Study Specific Intermediate Filament Proteins in Mammalian Systems -- 15. Hair Keratins and Hair Follicle-Specific Epithelial Keratins / Lutz Langbein, Herbert Spring, Michael A. Rogers, Silke Praetzel and Juergen Schweizer -- I. Introduction -- II. Procedures and Comments -- III. Pitfalls and Problems -- 16. Skin: An Ideal Model System to Study Keratin Genes and Proteins / Kelsie M. Bernot, Pierre A. Coulombe and Pauline Wong -- I. Introduction -- II. Collection of Mouse Skin Tissue for Analysis -- III. Preparation of Tissues for Morphological Studies -- IV. Cell Culture Studies -- V. Preparation and Analysis of Protein and RNA from Tissues and Cells -- VI. Pearls and Pitfalls -- VII. Conclusion -- 17. Studying Simple Epithelial Keratins in Cells and Tissues / Nam-On Ku, Diana M. Toivola, Qin Zhou, Guo-Zhong Tao, Bihui Zhong and M. Bishr Omary -- I. Introduction -- II. Materials and Methods -- III. Pearls and Pitfalls -- IV. Concluding Remarks -- 18. Muscle Intermediate Filament Proteins / Richard M. Robson, Ted W. Huiatt and Robert M. Bellin -- I. Introduction -- II. Materials -- III. Procedures -- IV. Pearls and Pitfalls -- V. Concluding Remarks -- 19. [superscript 35 S]Methionine Metabolic Labeling to Study Axonal Transport of Neuronal Intermediate Filament Proteins In Vivo / Stephanie Millecamps and Jean-Pierre Julien -- I. Introduction -- II. Materials and Instrumentation -- III. Procedures -- IV. Comments -- V. Pitfalls -- 20. Lamins / Georg Krohne -- I. Introduction -- II. Materials -- III. Purification of Lamins Produced in Bacteria -- IV. Production of Lamins in Insect Cells Using the Baculovirus System -- V. Selection of Cultured Cells and Tissues for Lamin Enrichment -- VI. Enrichment of Lamins from Isolated Mammalian Liver Nuclei -- VII. Enrichment of Lamins from Cultured Cells (Tested for Xenopus A6 Cells and HeLa Cells) -- VIII. Immunoprecipitation of Lamin from Cell Extracts -- 21. The Intermediate Filament Systems in the Eye Lens / Ming Der Perng, Aileen Sandilands, Jer Kuszak, Ralf Dahm, Alfred Wegener, Alan R. Prescott and Roy A. Quinlan -- I. Introduction -- II. Materials and Instrumentation -- III. Procedures -- IV. Pearls and Pitfalls -- V. Discussion and Concluding Remarks -- Part IV. Methods to Study Specific Intermediate Filament Proteins in Non-Mammalian Systems -- 22. Fish Keratins / Michael Schaffeld and Jurgen Markl -- I. Introduction -- II. Materials, Procedures, and Pitfalls -- III. Discussion and Concluding Remarks -- 23. Performing Functional Studies of Xenopus laevis Intermediate Filament Proteins Through Injection of Macromolecules into Early Embryos / Christine Gervasi and Ben G. Szaro -- I. Introduction -- II. Instrumentation and Materials -- III. Procedures -- IV. Pearls and Pitfalls -- V. Discussion and Concluding Remarks -- 24. Intermediate Filaments in Caenorhabditis elegans / Alexandra Fridkin, Anton Karabinos and Yosef Gruenbaum -- I. Introduction -- II. Intermediate Filaments in C. elegans -- III. General Methodology -- IV. Forming Intermediate Filaments In Vitro -- V. Indirect Immunofluorescence Analysis -- VI. Immunogold-Electron Microscopy Preembedding Staining of C. elegans Embryos -- VII. Sample Preparation for Transmission Electron Microscopy Analysis of C. elegans Embryos -- VIII. Live Imaging of C. elegans -- Part V. Methods to Study Intermediate Filament Associated Proteins -- 25. Plectin / Gunther A. Rezniczek, Lubomir Janda and Gerhard Wiche -- I. Introduction -- II. Materials and Methods -- III. Pearls and Pitfalls -- IV. Concluding Remarks -- 26. In Vitro Methods for Investigating Desmoplakin-Intermediate Filament Interactions and Their Role in Adhesive Strength / Tracie Y. Hudson, Lionel Fontao, Lisa M. Godsel, Hee-Jung Choi, Arthur C. Huen, Luca Borradori, William I. Weis and Kathleen J. Green -- I. Introduction -- II. Materials and Methods -- III. Pitfalls and Pearls -- IV. Discussion --
• 27. Studying Cytolinker Proteins / Dmitry Goryunov, Conrad L. Leung and Ronald K. H. Liem -- I. Introduction -- II. Methods for Studying Cytolinker Proteins -- III. Pearls and Pitfalls -- IV. Concluding Remarks -- 28. Keratin Bundling Proteins / Pawel Listwan and Joseph A. Rothnagel -- I. Introduction -- II. Methods -- III. Pearls and Pitfalls -- IV. Concluding Remarks -- 29. Lamin-Associated Proteins / Cecilia Ostlund and Howard J. Worman -- I. Introduction -- II. Materials -- III. Procedures -- IV. Pearls and Pitfalls -- V. Discussion and Concluding Remarks.

ISBN

• 0125641737 (alk. paper)
• 9780125641739 (alk. paper)

OCLC

• ocm57638763
• 57638763
• SCSB-5359364

Owning Institutions

Columbia University Libraries