Research Catalog

Title

Hedgehog signaling protocols / edited by Jamila I. Horabin.

Publication

Totowa, N.J. : Humana ; Paisley : Quantum [distributor], 2007.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Horabin, Jamila I.

Description

xvii, 256 pages : illustrations (some color); 24 cm.

Series Statement

Methods in molecular biology ; 397

Uniform Title

Methods in molecular biology ; v. 397.

Subject

• Cellular signal transduction > Methodology
• Transcription factors > Methodology
• Signal Transduction
• Transcription Factors
• Research

Contents

1. Purifying the Hedgehog Protein and its Variants / Darren P. Baker, Frederick R. Taylor and R. Blake Pepinsky -- 2. Application of Sonic Hedgehog to the Developing Chick Limb / Eva Tiecke and Cheryll Tickle -- 3. Manipulation of Hedgehog Signaling in Xenopus by Means of Embryo Microinjection and Application of Chemical Inhibitors / Thomas Hollemann, Emmanuel Tadjuidje, Katja Koebernick and Tomas Pieler -- 4. Isolation of Rat Telencephalic Neural Explants to Assay Shh GABAergic Interneuron Differentiation-Inducing Activity / Rina Mady and Jhumku D. Kohtz -- 5. Genetic Analysis of the Vertebrate Hedgehog-Signaling Pathway Using Muscle Cell Fate Specification in the Zebrafish Embryo / Sudipto Roy -- 6. Efficient Manipulation of Hedgehog/GLI Signaling Using Retroviral Expression Systems / Maria Kasper, Gerhard Regl, Thomas Eichberger, Anna-Maria Frischauf and Fritz Aberger -- 7. Cell Surface Marker and Cell Cycle Analysis, Hedgehog Signaling, and Flow Cytometry / Kristina Detmer and Ronald E. Garner -- 8. Detecting Tagged Hedgehog with Intracellular and Extracellular Immunocytochemistry for Functional Analysis / Ainhoa Callejo, Luis Quijada and Isabel Guerrero -- 9. Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled with Fluorescent in situ Hybridization of Hedgehog Target Genes / Armel Gallet and Pascal P. Therond -- 10. RNAi in the Hedgehog Signaling Pathway: pFRiPE, a Vector for Temporally and Spatially Controlled RNAi in Drosophila / Eric Marois and Suzanne Eaton -- 11. Germline Clone Analysis for Maternally Acting Drosophila Hedgehog Components / Erica M. Selva and Beth E. Stronach -- 12. Clonal Analysis of Hedgehog Signaling in Drosophila Somatic Tissues / Christine M. Bankers and Joan E. Hooper -- 13. GAL4/UAS Targeted Gene Expression for Studying Drosophila Hedgehog Signaling / Denise Busson and Anne-Marie Pret -- 14. Biochemical Fractionation of Drosophila Cells / Melanie Stegman and David Robbins -- 15. Using Immunoprecipitation to Study Protein-Protein Interactions in the Hedgehog-Signaling Pathway / Chao Tong and Jin Jiang -- 16. Sequence Analyses to Study the Evolutionary History and Cis-Regulatory Elements of Hedgehog Genes / Ferenc Muller and Anne-Gaelle Borycki.

ISBN

• 9781588296924 (hbk.)
• 158829692X (hbk.)

OCLC

• ocn123798709
• 123798709
• SCSB-5365146

Owning Institutions

Columbia University Libraries