Research Catalog

Title

Exocytosis and endocytosis / [edited by] Andrei I. Ivanov.

Publication

Totowa, N.J. : Humana Press, [2008], ©2008.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Ivanov, Andrei I.

Description

xv, 412 pages : illustrations; 25 cm.

Series Statement

Methods in molecular biology ; 440

Uniform Title

Methods in molecular biology (Clifton, N.J.) ; v. 440.

Subject

• Exocytosis > Laboratory manuals
• Endocytosis > Laboratory manuals
• Exocytosis
• Endocytosis
• Cytological Techniques

Genre/Form

Laboratory Manual.

Bibliography (note)

• Includes bibliographical references and index.

Contents

Part I. An Overview of Methods for Analyzing Vesicular Trafficking -- 1. Vesicular Trafficking: Molecular Tools and Targets / Elena V. Vassilieva and Asma Nusrat -- 2. Pharmacological Inhibition of Endocytic Pathways: Is It Specific Enough to Be Useful? / Andrei I. Ivanov -- Part II. Cell-Free and Biochemical Assays of Exocytosis and Endocytosis -- 3. In Vitro Assays to Measure SNARE-Mediated Vesicle Fusion / Susanne Kreye, Jorg Malsam and Thomas H. Sollner -- 4. Imaging Ca[superscript 2+]-Triggered Exocytosis of Single Secretory Granules on Plasma Membrane Lawns from Neuroendocrine Cells / Thorsten Lang -- 5. FRAP Analysis of Secretory Granule Lipids and Proteins in the Sea Urchin Egg / Julian L. Wong and Gary M. Wessel -- 6. A Cell-Free Assay for Endocytosis of E-Cadherin / Toshiaki Sakisaka and Yoshimi Takai -- 7. Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin / Noriyuki Nishimura and Takuya Sasaki -- 8. Fractionation of Subcellular Membrane Vesicles of Epithelial and Nonepithelial Cells by OptiPrep Density Gradient Ultracentrifugation / Xuhang Li and Mark Donowitz -- 9. Identification and Characterization of Interacting Partners of Rab GTPases by Yeast Two-Hybrid Analyses / Mark Kail and Angelika Barnekow -- Part III. Analysis of Endocytosis and Exocytosis in Different Cell Systems -- 10. High-Throughput Analysis of the Dynamics of Recycling Cell Surface Proteins / Roland Govers, David E. James and Adelle C.F. Coster -- 11. Studying Phagocytosis by Live-Cell Scintillation Proximity Assay / Walter Stockinger and Axel Nohturfft -- 12. Transcytosis of Polymeric Immunoglobulin A in Polarized Madin-Darby Canine Kidney Cells / Asli Oztan, Christine Rondanino and Gerard Apodaca -- 13. The Use of Syntaxin Chimeras to Study Polarized Protein Trafficking in Epithelial Cells / Martin B.A. ter Beest -- 14. Targeting the Epithelial SNARE Machinery by Bacterial Neurotoxins / Veronique Proux-Gillardeaux and Thierry Galli -- 15. Exocytosis of Endothelial Cells Is Regulated by N-Ethylmaleimide-Sensitive Factor / Munekazu Yamakuchi, Marcella Ferlito, Craig N. Morrell, Kenji Matsushita, Craig A. Fletcher, Wangsen Cao and Charles J. Lowenstein -- 16. Molecular Dissection of HCl Secretion in Gastric Parietal Cells Using Streptolysin O Permeabilization / Xia Ding, Fang Wu, Zhen Guo and Xuebiao Yao -- 17. Addressing Membrane Protein Topology Using the Fluorescence Protease Protection (FPP) Assay / Holger Lorenz, Dale W. Hailey and Jennifer Lippincott-Schwartz -- 18. Visualizing Clathrin-Mediated IgE Receptor Internalization by Electron and Atomic Force Microscopy / Alan R. Burns, Janet M. Oliver, Janet R. Pfeiffer and Bridget S. Wilson -- 19. Analyzing Endosomes in Nonsectioned Cells by Transmission Electron Microscopy / Willem Stoorvogel -- 20. Imaging Exocytosis of Single Insulin Secretory Granules With TIRF Microscopy / Shinya Nagamatsu and Mica Ohara-Imaizumi -- 21. Reconstitution of Depolarization and Ca[superscript 2+]-Evoked Secretion in Xenopus Oocytes Monitored by Membrane Capacitance / Roy Cohen, Bernhard M. Schmitt and Daphne Atlas -- 22. Low-Noise Recording of Single-Vesicle Capacitance Steps in Cell-Attached Patches / Vitaly Klyachko, Zhenjie Zhang and Meyer Jackson -- 23. Good Practices in Single-Cell Amperometry / David J. Machado, Monica S. Montesinos and Ricardo Borges -- 24. Analysis of Single-Vesicle Exocytotic Events Recorded by Amperometry / Eugene V. Mosharov -- Part IV. Analysis of Exocytosis and Endocytosis in Model Organisms -- 25. Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in Caenorhabditis elegans / Dmitry Poteryaev and Anne Spang -- 26. FM 1-43 Labeling of Synaptic Vesicle Pools at the Drosophila Neuromuscular Junction / Patrik Verstreken, Tomoko Ohyama and Hugo J. Bellen -- 27. Probing E-Cadherin Endocytosis by Morpholino-Mediated Rab5 Knockdown in Zebrafish / Florian Ulrich and Carl-Philipp Heisenberg -- 28. Quantifying Endocytosis In Vivo Using Intravital Two-Photon Microscopy / Ruben M. Sandoval and Bruce A. Molitoris.

ISBN

• 9781588298652 (alk. paper)
• 1588298655 (alk. paper)

LCCN

2007933723

OCLC

• ocn178311076
• 178311076
• SCSB-5382109

Owning Institutions

Columbia University Libraries