Research Catalog

Title

Organelle proteomics / edited by Delphine Pflieger and Jean Rossier.

Publication

Totowa, NJ : Humana ; London : Springer [distributor], [2008], ©2008.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Pflieger, Delphine.
• Rossier, Jean.

Description

xvii, 421 pages : illustrations; 25 cm.

Series Statement

Methods in molecular biology, 1064-3745 ; 432

Uniform Title

Methods in molecular biology (Clifton, N.J.) ; v. 432.

Subject

• Cell organelles
• Proteomics
• Organelles
• Proteomics > methods
• Cells

Genre/Form

Laboratory Manual.

Bibliography (note)

• Includes bibliographical references and index.

Contents

1. Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography-Tandem Mass Spectrometry / Edwin P. Romijn and John R. Yates III -- Part I. Analysis of Organelles, Vesicles, and Protein Machineries -- 2. Purification and Proteomic Analysis of Chloroplasts and their Sub-Organellar Compartments / Daniel Salvi, Norbert Rolland, Jacques Joyard and Myriam Ferro -- 3. Proteomic Analysis of the Stacked Golgi Complex / Mark S. Lowenthal, Kathryn E. Howell and Christine C. Wu -- 4. Purification of Saccharomyces cerevisiae Mitochondria by Zone Electrophoresis in a Free Flow Device / Hans Zischka, Norbert Kinkl, Ralf J. Braun and Marius Ueffing -- 5. Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria: Activity Measurement and Subunit Composition Analysis / Claire Lemaire and Genevieve Dujardin -- 6. Mitochondrial Proteomics: Analysis of a Whole Mitochondrial Extract with Two-Dimensional Electrophoresis / Thierry Rabilloud -- 7. Purification and Proteomic Analysis of the Mouse Liver Mitochondrial Inner Membrane / Sandrine Da Cruz and Jean-Claude Martinou -- 8. Subcellular Fractionation and Proteomics of Nuclear Envelopes / Laurence Florens, Nadia Korfali and Eric C. Schirmer -- 9. Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction / Tsuneyoshi Horigome, Kazuhiro Furukawa and Kohei Ishii -- 10. Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis / Kiichi Fukui, Hideaki Takata and Susumu Uchiyama -- 11. Purification and Proteomic Analysis of Plant Plasma Membranes / Erik Alexandersson, Niklas Gustavsson, Katja Bernfur, Adine Karlsson, Per Kjellbom and Christer Larsson -- 12. Protocol to Enrich and Analyze Plasma Membrane Proteins from Frozen Tissues / Jacek R. Wisniewski -- 13. Proteomic Characterization of Membrane Protein Topology / Adele R. Blackler and Christine C. Wu -- 14. Free Flow Isoelectric Focusing: A Method for the Separation of Both Hydrophilic and Hydrophobic Proteins of Rat Liver Peroxisomes / Markus Islinger and Gerhard Weber -- 15. Use of Gas-Phase Fractionation to Increase Protein Identifications: Application to the Peroxisome / Jacob Kennedy and Eugene C. Yi -- 16. Purification and Proteomic Analysis of Lysosomal Integral Membrane Proteins / Huiwen Zhang, Xiaolian Fan, Rick Bagshaw, Don J. Mahuran and John W. Callahan -- 17. Affinity Purification of Soluble Lysosomal Proteins for Mass Spectrometric Identification / Sylvie Kieffer-Jaquinod, Agnes Chapel, Jerome Garin and Agnes Journet -- 18. Purification and Proteomic Analysis of Synaptic Vesicles / Holly D. Cox and Charles M. Thompson -- 19. Purification and Proteomics Analysis of Pancreatic Zymogen Granule Membranes / Xuequn Chen and Philip C. Andrews -- 20. Isolation and Proteomic Analysis of Chlamydomonas Centrioles / Lani C. Keller and Wallace F. Marshall -- 21. Purification and Proteomic Analysis of 20S Proteasomes from Human Cells / Marie-Pierre Bousquet-Dubouch, Sandrine Uttenweiler-Joseph, Manuelle Ducoux-Petit, Mariette Matondo, Bernard Monsarrat and Odile Burlet-Schiltz -- 22. Characterization of E. coli Ribosomal Particles: Combined Analysis of Whole Proteins by Mass Spectrometry and of Proteolytic Digests by Liquid Chromatography-Tandem Mass Spectrometry / Isabelle Iost, Julie Charollais, Joelle Vinh and Delphine Pflieger -- Part II. Proteins Identified in the Organelle Sample: True Components or Contaminants? -- 23. Assessment of Organelle Purity Using Antibodies and Specific Assays: The Example of the Chloroplast Envelope / Daniel Salvi, Norbert Rolland, Jacques Joyard and Myriam Ferro -- 24. Identifying Bona Fide Components of an Organelle by Isotope-Coded Labeling of Subcellular Fractions: An Example in Peroxisomes / Marcello Marelli, Alexey I. Nesvizhskii and John D. Aitchison -- 25. Determination of Genuine Residents of Plant Endomembrane Organelles using Isotope Tagging and Multivariate Statistics / Kathryn S. Lilley and Tom P. J. Dunkley -- 26. Quantitative Proteomic Analysis to Profile Dynamic Changes in the Spatial Distribution of Cellular Proteins / Wei Yan, Daehee Hwang and Ruedi Aebersold -- 27. Use of Transcriptomic Data to Support Organelle Proteomic Analysis / Wallace F. Marshall.

ISBN

• 9781588297792 (hbk.)
• 1588297799 (hbk.)

LCCN

2007941655

OCLC

• ocn178312727
• 178312727
• SCSB-5391897

Owning Institutions

Columbia University Libraries