Research Catalog

Title

Molecular beacons : signalling nucleic acid probes, methods, and protocols / edited by Andreas Marx and Oliver Seitz.

Publication

Totowa, N.J. : Humana ; London : Springer [distributor], 2008.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Marx, Andreas.
• Seitz, Oliver.

Description

xii, 266 pages : illustrations (some color); 24 cm.

Series Statement

Methods in molecular biology ; 429

Uniform Title

Methods in molecular biology ; v. 429.

Subjects

• Fluorescent probes
• Molecular biology
• Molecular Biology
• Cellular signal transduction
• Fluorescent Dyes
• Signal Transduction

Bibliography (note)

• Includes bibliographical references and index.

Contents

I. Introduction -- 1. Basic Principles of Fluorescence and Energy Transfer / Larry E. Morrison -- II. Applications -- 2. Multiplex Detection of Mutations / David S. Perlin, Sergey Balashov and Steven Park -- 3. Detection of Salmonella Species in Foodstuffs / Arvind A. Bhagwat, Jitu Patel, Trina Chua, Audrey Chan, Saul Ruiz Cruz and Gustavo A. Gonzalez Aguilar -- 4. Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR / Gunter Schmidtke and Marcus Groettrup -- 5. Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma / Sebastian Lukasiak, Kai Breuhahn, Claudia Schiller, Gunter Schmidtke and Marcus Groettrup -- 6. Absolute Quantitation of Feline Leukemia Virus Proviral DNA and Viral RNA Loads by TaqMan Real-Time PCR and RT-PCR / Valentino Cattori and Regina Hofmann-Lehmann -- 7. High-Throughput Real-Time PCR / Thomas D. Schmittgen, Eun Joo Lee and Jinmai Jiang -- 8. Design and Use of Scorpions Fluorescent Signalling Molecules / Rachael Carters, Jennifer Ferguson, Rupert Gaut, Paul Ravetto, Nicola Thelwell and David Whitcombe -- 9. Hybridization Probe Pairs and Single-Labeled Probes: an Alternative Approach for Genotyping and Quantification / Thomas Froehlich and Oliver Geulen -- III. New Technologies -- 10. EasyBeacons for the Detection of Methylation Status of Single CpG Duplets / Ulf B. Christensen -- 11. Quenched Autoligation Probes / Adam P. Silverman, Hiroshi Abe and Eric T. Kool -- 12. HyBeacon Probes for Rapid DNA Sequence Detection and Allele Discrimination / David J. French, David G. McDowell, Paul Debenham, Nittaya Gale and Tom Brown -- 13. FIT-Probes in Real-Time PCR / Elke Socher and Oliver Seitz -- 14. SNP Genotyping by Unlabeled Probe Melting Analysis / Maria Erali, Robert Palais and Carl Wittwer -- IV. Non-Nucleic Acid Targets -- 15. Molecular Beacons for Protein-DNA Interaction Studies / Jun Li, Zehui Charles Cao, Zhiwen Tang, Kemin Wang and Weihong Tan -- 16. DNA Polymerase Profiling / D. Summerer -- 17. Modular Reporter Hairpin Ribozymes for Analyzing Molecular Interactions / S. Hani Najafi-Shoushtari and Michael Famulok -- 18. Screening of Molecular Interactions Using Reporter Hammerhead Ribozymes / Jorg S. Hartig and Michael Famulok.

ISBN

• 9781588297006 (hbk.)
• 1588297004 (hbk.)

OCLC

• ocn166372361
• 166372361
• SCSB-5398161

Owning Institutions

Columbia University Libraries