Research Catalog

Drosophila : methods and protocols

Title
Drosophila : methods and protocols / edited by Christian Dahmann.
Publication
Totowa, N.J. : Humana ; London : Springer [distributor], 2008.

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TextRequest in advance QH506 .M56 v.420Off-site

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Details

Additional Authors
Dahmann, Christian.
Description
xi, 437 pages : illustrations; 24 cm.
Series Statement
Methods in molecular biology ; v. 420
Uniform Title
Methods in molecular biology ; v. 420.
Subject
  • Drosophila
  • Molecular biology > Research
  • Molecular biology > Technique
  • Animal models in research
  • Molecular Biology
  • Models, Animal
  • Research
Contents
1. Drosophila melanogaster and the Development of Biology in the 20th Century / Alfonso Martinez Arias -- 2. Getting Started: An Overview on Raising and Handling Drosophila / Hugo Stocker and Peter Gallant -- 3. FlyBase: A Database for the Drosophila Research Community / Rachel Drysdale / the FlyBase Consortium -- 4. The Use of P-Element Transposons to Generate Transgenic Flies / Andre Bachmann and Elisabeth Knust -- 5. The GAL4 System: A Versatile System for the Expression of Genes / David A. Elliott and Andrea H. Brand -- 6. P-Element Mutagenesis / Thomas Hummel and Christian Klambt -- 7. EMS Screens: From Mutagenesis to Screening and Mapping / Christian Bokel -- 8. RNAi Screening in Cultured Drosophila Cells / Sandra Steinbrink and Michael Boutros -- 9. Methods for Homologous Recombination in Drosophila / Keith A. Maggert, Wei J. Gong and Kent G. Golic -- 10. Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis / Johannes Bischof and Konrad Basler -- 11. Cuticle Preparation of Drosophila Embryos and Larvae / Cyrille Alexandre -- 12. Immunolabeling of Embryos / H.-Arno J. Muller -- 13. Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins / Matthieu Cavey and Thomas Lecuit -- 14. Analysis of Neuromuscular Junctions: Histology and In Vivo Imaging / Andreas Schmid and Stephan J. Sigrist -- 15. Immunolabeling of Imaginal Discs / Thomas Klein -- 16. Imaging Drosophila Pupal Wing Morphogenesis / Anne-Kathrin Classen, Benoit Aigouy, Angela Giangrande and Suzanne Eaton -- 17. Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections / Konstantin Gaengel and Marek Mlodzik -- 18. Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues / Eric Lecuyer, Neela Parthasarathy and Henry M. Krause -- 19. Linear RNA Amplification for the Production of Microarray Hybridization Probes / Ansgar Klebes and Thomas B. Kornberg -- 20. An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs / Nicholas S. Sokol -- 21. Extraction and Immunoblotting of Proteins From Embryos / Andreas Wodarz -- 22. Purification of Drosophila Protein Complexes for Mass Spectrometry / Christoph Juschke and Jurgen A. Knoblich -- 23. Mass Production of Drosophila Embryos and Chromatographic Purification of Native Protein Complexes / Natascha Kunert and Alexander Brehm -- 24. Flow Cytometric Analysis of Drosophila Cells / Aida Flor A. de la Cruz and Bruce A. Edgar -- 25. Drosophila Cell Lines as Model Systems and as an Experimental Tool / Buzz Baum and Lucy Cherbas -- Appendix. Phenotypic Markers in Drosophila / Jennifer Childress and Georg Halder.
ISBN
  • 9781588298171 (hbk.)
  • 1588298175 (hbk.)
OCLC
  • ocn165410474
  • 165410474
  • SCSB-5399440
Owning Institutions
Columbia University Libraries