Research Catalog
RNAi : design and application
- Title
- RNAi : design and application / edited by Sailen Barik.
- Publication
- Totowa, NJ : Humana Press, [2008], ©2008.
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| Status | Format | Access | Call Number | Item Location |
|---|---|---|---|---|
| Text | Request in advance | QH506 .M56 v.442 | Off-site |
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Details
- Additional Authors
- Barik, Sailen.
- Description
- xiv, 270 pages : illustrations (some color); 24 cm.
- Summary
- "RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology. In RNAi: Design and Applications, leaders in the field contribute methods and bench protocols designed for practical, everyday use of RNAi in biological research. Divided into two parts, this volume covers fundamentals including designs of RNAi, biochemical assay protocols for the major components of RNAi, and study of potential off-target effects, followed by an extensive section covering various applications of RNAi in diverse model organisms and systems, from antiviral and anticancer applications to altering flower color in plants."--BOOK JACKET.
- Series Statement
- Methods in molecular biology, 1064-3745 ; 442
- Uniform Title
- Methods in molecular biology (Clifton, N.J.) ; v. 442.
- Subjects
- Genre/Form
- Laboratory Manual.
- Bibliography (note)
- Includes bibliographical references and index.
- Contents
- Pt. I. Designing Optimal RNAi Tools -- 1. Principles of Dicer Substrate (D-siRNA) Design and Function / Mohammed Amarzguioui and John J. Rossi -- 2. Expression, Purification, and Analysis of Recombinant Drosophila Dicer-1 and Dicer-2 Enzymes / Xuecheng Ye and Qinghua Liu -- 3. In vitro RNA Cleavage Assay for Argonaute-Family Proteins / Keita Miyoshi, Hiroshi Uejima, Tomoko Nagami-Okada, Haruhiko Siomi and Mikiko C. Siomi -- 4. Identifying siRNA-Induced Off-Targets by Microarray Analysis / Emily Anderson, Queta Boese, Anastasia Khvorove and Jon Karpilow -- Pt. II. Application of RNAi in Diverse Organisms -- 5. Hydrodynamic Delivery of siRNA in a Mouse Model of Sepsis / Doreen E. Wesche-Soldato, Joanne Lomes-Nelre, Mario Perl, Chun-Shiang Chung and Alfred Ayala -- 6. Nasal Delivery of siRNA / Vira Bitko and Sailen Barik -- 7. RNA Interference as a Genetic Tool in Trypanosomes / Vivian Bellofatto and Jennifer B. Palenchar -- 8. Lentivirus-Mediated RNA Interference in Mammalian Neurons / Scott Q. Harper and Pedro Gonzalez-Alegre -- 9. Silencing Genes by RNA Interference in the Protozoan Parasite Entamoeba histolytica / Carlos F. Solis and Nancy Guillen -- 10. Use of RNAi in C. elegans / Tsuyoshi Ohkumo, Chikahide Masutani, Toshihiko Eki and Fumio Hanaoka -- 11. Application of siRNA Against SARS in the Rhesus Macaque Model / Qingquan Tang, Baojian Li, Martin Woodle and Patrick Y. Lu -- 12. siRNA and shRNA as Anticancer Agents in a Cervical Cancer Model / Wenyi Gu, Lisa Putrel and Nigel McMillan -- 13. Identification and Expression Analysis of Small RNAs During Development / Toshiaki Watanabe, Hiroshi Imai and Naojiro Minami -- 14. Screening and Identification of Virus-Encoded RNA Silencing Suppressors / Sumona Karjee, Mohammad Nurul Islam and Sunil K. Mukherjee -- 15. Application of RNA Interference in Functional Genomics Studies of a Social Insect / Michael E. Scharf, Xuguo Zhou and Margaret A. Schwinghammer -- 16. Temporal Control of Gene Silencing by in ovo Electroporation / Thomas Baeriswyl, Olivier Meuti and Esther T. Stoeckli -- 17. Altering Flower Color in Transgenic Plants by RNAi-Mediated Engineering of Flavonoid Biosynthetic Pathway / Yoshikazu Tanaka, Noriko Nakamura and Junichi Togami -- 18. Transgenic RNA Interference in Mice / Pumin Zhang.
- ISBN
- 9781588298744 (alk. paper)
- 1588298744 (alk. paper)
- LCCN
- 2007940759
- OCLC
- ocn172979420
- ocn227125413
- 227125413
- 172979420
- SCSB-5403783
- Owning Institutions
- Columbia University Libraries