Research Catalog

Title

Auditory and vestibular research : methods and protocols / edited by Bernd Sokolowski.

Publication

New York, N.Y. : Humana ; London : Springer [distributor], [2009], ©2009.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Sokolowski, Bernd.

Description

xii, 421 pages : illustrations (some color); 27 cm.

Series Statement

• Springer protocols
• Methods in molecular biology, 1064-3745 ; 493

Uniform Title

• Methods in molecular biology (Clifton, N.J.) ; v. 493.
• Springer protocols (Series)

Subjects

• Vestibule, Labyrinth
• Vestibule, Labyrinth > physiology
• Vestibular apparatus
• Hearing Disorders
• Auditory Pathways > physiology
• Vestibular Diseases > diagnosis
• Equilibrium (Physiology)
• Ear Diseases > diagnosis
• Hearing disorders

Bibliography (note)

• Includes bibliographical references and index.

Contents

1. RNA isolation from Xenopus inner ear sensory endorgans for transcriptional profiling and molecular cloning / Casilda Trujillo-Provencio, TuShun R. Powers, David R. Sultemeier and Elba E. Serrano -- 2. Synthesis of biotin-labeled RNA for gene expression measurements using oligonucleotide arrays / Ana E. Vazquez, Liping Nie and Ebenezer N. Yamoah -- 3. In situ hybridization approach to study mRNA expression and distribution in cochlear frozen sections / Hakim Hiel -- 4. Lineage analysis of inner ear cells using genomic tags for clonal identification / Takunori Satoh and Donna M. Fekete -- 5. Genetic fate-mapping approaches : new means to explore the embryonic origins of the cochlear nucleus / Jun Chul Kim and Susan M. Dymecki -- 6. The practical use of Cre and laxP technologies in mouse auditory research / Yiling Yu and Jian Zuo -- 7. Helios gene gun-mediated transfection of the inner ear sensory epithelium / Inna A. Belyantseva 103 -- 8. Electroporation-mediated gene transfer to the developing mouse inner ear / John V. Brigande, Samuel P. Gubbels, David W. Woessner, Jonathan J. Jungwirth and Catherine S. Bresee -- 9. Isolation of sphere-forming stem cells from the mouse inner ear / Kazuo Oshima, Pascal Senn and Stefan Heller -- 10. Molecular biology of vestibular schwannomas / Long-Sheng Chang and D. Bradley Welling -- 11. Multilocus sequence typing and pulsed field gel electrophoresis of otitis media causing pathogens / Jonathan C. Thomas and Melinda M. Pettigrew -- 12. Fluorescence "in situ" hybridization for the detection of biofilm in the middle ear and upper respiratory trace mucosa / Laura Nistico, Armin Gieseke, Paul Stoodley, Luanne Hall-Stoodley, Joseph E. Kerschner and Garth D. Ehrlich -- 13. Positional cloning of deafness genes / Hannie Kremer and Frans P. M. Cremers -- 14. Twist-off purification of hair bundles / Jung-Bum Shin, James Pagana and Peter G. Gillespie -- 15. Yeast two-hybrid screening to test for protein-protein interactions in the auditory system / Dhasakumar S. Navaratnam -- 16. The use of 2-D gels to identify novel protein-protein interactions in the cochlea / Thandavarayan Kathiresan, Margaret C. Harvey and Bernd H. A. Sokolowski -- 17. Identification of functionally important residues/domains in membrane proteins using an evolutionary approach coupled with systematic mutational analysis / Lavanya Rajagopalan, Fred A. Pereira, Olivier Lichtarge and William E. Brownell -- 18. In vivo verification of protein interactions in the inner ear by coimmunoprecipitation / Margaret C. Harvey and Bernd H. A. Sokolowski -- 19. Identification of transcription factor-DNA interactions using chromation immunoprecipitation assays / Liping Nie, Ana E. Vazquez and Ebenezer N. Yamoah -- 20. Surface plasmon resonance (SPR) analysis of binding interactions of proteins in inner-ear sensory epithelia / Dennis G. Drescher, Neeliyath A. Ramakrishnan and Marian J. Drescher -- 21. Multiplexed isobaric tagging protocols for quantitative mass spectrometry approaches to auditory research / Douglas E. Vetter, Johnvesly Basappa and Sevin Turcan -- 22. Fluorescence microscopy methods in the study of protein structure and function / Heather Jensen-Smith, Benjamin Currall, Danielle Rossino, LeAnn Tiede, Michael Nichols and Richard Hallworth -- 23. Ion imaging in the cochlear hair cells / Gregory I. Frolenkov -- 24. Atomic force microscopy in studies of the cochlea / Michio Murakoshi and Hiroshi Wada.

ISBN

• 9781934115626 (hbk.)
• 1934115622 (hbk.)

LCCN

2008934544

OCLC

• ocn233933530
• 233933530
• SCSB-5436586

Owning Institutions

Columbia University Libraries