Research Catalog

Title

Mycobacteria protocols / edited by Tanya Parish and Amanda Claire Brown.

Publication

Totowa, N.J. : Humana ; London : Springer [distributor], 2008.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Parish, Tanya.
• Brown, Amanda Claire.

Description

xix, 456 pages : illustrations (some color); 25 cm.

Series Statement

Methods in molecular biology ; v. 465

Uniform Title

Methods in molecular biology ; v. 465.

Subject

• Mycobacteria > Laboratory manuals
• Mycobacterium
• Genetic Techniques
• Molecular Biology > methods
• Cytological Techniques

Genre/Form

Laboratory Manual.

Contents

1. Isolation of Mycobacterium Species Genomic DNA / John T. Belisle, Spencer B. Mahaffey and Preston J. Hill -- 2. Isolation of Mycobacterial RNA / Tige R. Rustad, David M. Roberts, Reiling P. Liao and David R. Sherman -- 3. Lipoglycans of Mycobacterium tuberculosis: Isolation, Purification, and Characterization / Libin Shi, Jordi B. Torrelles and Delphi Chatterjee -- 4. Analyzing Lipid Metabolism: Activation and [beta]-Oxidation of Fatty Acids / Paul Robert Wheeler -- 5. Analysis of Lipid Biosynthesis and Location / Paul Robert Wheeler -- 6. Whole Genome Analysis Using Microarrays / S. J. Waddell, J. Hinds and P. D. Butcher -- 7. Use of DNA Microarrays to Study Global Patterns of Gene Expression / Roberta Provvedi, Giorgio Palu and Riccardo Manganelli -- 8. Two-Dimensional Gel Electrophoresis-Based Proteomics of Mycobacteria / Jens Mattow, Frank Siejak, Kristine Hagens, Julia Kreuzeder, Stefan H. E. Kaufmann and Ulrich E. Schaible -- 9. Transport Assays and Permeability in Pathogenic Mycobacteria / Marie-Antoinette Laneelle and Mamadou Daffe -- 10. Continuous Culture of Mycobacteria / Joanna Bacon and Kim A. Hatch -- 11. Measuring Minimum Inhibitory Concentrations in Mycobacteria / Frederick A. Sirgel, Ian J. F. Wiid and Paul D. van Helden -- 12. Rapid Screening of Inhibitors of Mycobacterium tuberculosis Growth Using Tetrazolium Salts / Anita G. Amin, Shiva K. Angala, Delphi Chatterjee and Dean C. Crick -- 13. Electroporation of Mycobacteria / Renan Goude and Tanya Parish -- 14. Ins and Outs of Mycobacterial Plasmids / Farahnaz Movahedzadeh and Wilbert Bitter -- 15. The Use of Temperature-Sensitive Plasmids in Mycobacteria / Damien Portevin, Wladimir Malaga and Christophe Guilhot -- 16. Heterologous Expression of Genes in Mycobacteria / James A. Triccas and Anthony A. Ryan -- 17. Inducible Expression Systems for Mycobacteria / Christopher M. Sassetti -- 18. Assaying Promoter Activity Using LacZ and GFP as Reporters / Paul Carroll and Jade James -- 19. Construction of Unmarked Deletion Mutants in Mycobacteria / Houhui Song, Frank Wolschendorf and Michael Niederweis -- 20. Construction of Targeted Mycobacterial Mutants by Homologous Recombination / Sharon L. Kendall and Rosangela Frita -- 21. Phage Transposon Mutagenesis / M. Sloan Siegrist and Eric J. Rubin -- 22. Gene Essentiality Testing in Mycobacterium smegmatis Using Specialized Transduction / Apoorva Bhatt and William R. Jacobs, Jr. -- 23. Gene Switching and Essentiality Testing / Amanda Claire Brown -- 24. Insertion Element IS6110-Based Restriction Fragment Length Polymorphism Genotyping of Mycobacterium tuberculosis / Robin M. Warren, Paul D. van Helden and Nicolaas C. Gey van Pittius -- 25. Typing Mycobacterium tuberculosis Using Variable Number Tandem Repeat Analysis / T. J. Brown, V. N. Nikolayevskyy and F. A. Drobniewski -- 26. Molecular Detection of Drug-Resistant Mycobacterium tuberculosis with a Scanning-Frame Oligonucleotide Microarray / Dmitriy V. Volokhov, Vladimir E. Chizhikov, Steven Denkin and Ying Zhang -- 27. MycoDB: An Online Database for Comparative Genomics of the Mycobacteria and Related Organisms / Roy R. Chaudhuri -- 28. Environmental Amoebae and Mycobacterial Pathogenesis / Melanie Harriff and Luiz E. Bermudez.

ISBN

• 9781588298898 (hbk.)
• 1588298892 (hbk.)

OCLC

• ocn222160271
• SCSB-5442944

Owning Institutions

Columbia University Libraries