Research Catalog

Title

Transgenic wheat, barley and oats : production and characterization protocols / edited by Huw D. Jones and Peter R. Shewry.

Publication

New York, NY : Humana Press, [2009], ©2009.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Jones, Huw D.
• Shewry, P. R. (Peter R.)

Description

x, 349 pages, 8 unnumbered pages of plates : illustrations (some color); 27 cm.

Series Statement

• Methods in molecular biology, 1064-3745 ; 478
• Springer protocols

Uniform Title

• Methods in molecular biology (Clifton, N.J.) ; v. 478.
• Springer protocols (Series)

Subjects

• Laboratory Manual
• Hordeum > genetics
• Oats > Genetics
• Transgenic plants
• Wheat > Genetics
• Barley > Genetics
• Avena > genetics
• Plants, Genetically Modified
• Triticum > genetics
• Genetic Engineering > methods

Genre/Form

Laboratory Manual.

Bibliography (note)

• Includes bibliographical references and index.

Contents

Pt. I. Introduction -- 1. Transgenic Wheat, Barley and Oats: Production and Characterization / Paul A. Lazzeri and Huw D. Jones -- Pt. II. Transformation and Regeneration -- 2. Selection of Transformed Plants / Huw D. Jones and Caroline A. Sparks -- 3. Reporter Genes / Alison Huttly -- 4. Biolistics Transformation of Wheat / Caroline A. Sparks and Huw D. Jones -- 5. Agrobacterium-Mediated Transformation of Bread and Durum Wheat Using Freshly Isolated Immature Embryos / Huixia Wu, Angela Doherty and Huw D. Jones -- 6. Floral Transformation of Wheat / Sujata Agarwal, Star Loar, Camille Steber and Janice Zale -- 7. Highly Efficient Agrobacterium-Mediated Transformation of Wheat via In Planta Inoculation / Thierry Risacher, Melanie Craze, Sarah Bowden, Wyatt Paul and Tina Barsby -- 8. Barley Transformation Using Biolistic Techniques / Wendy A. Harwood and Mark A. Smedley -- 9. Barley Transformation Using Agrobacterium-Mediated Techniques / Wendy A. Harwood, Joanne G. Bartlett, Silvia C. Alves, Matthew Perry, Mark A. Smedley, Nicola Leyland and John W. Snape -- 10. Transformation of Oats and Its Application to Improving Osmotic Stress Tolerance / Shahina B. Maqbool, Heng Zhong, Hesham F. Oraby and Mariam B. Sticklen -- Pt. III. Gene and Protein Expression -- 11. Promoter Sequences for Defining Transgene Expression / Huw D. Jones and Caroline A. Sparks -- 12. Down-Regulation of Gene Expression by RNA-Induced Gene Silencing / Silvia Travella and Beat Keller -- Pt. IV. Characterisation of Transgenic Plants -- 13. Gene Insertion Patterns and Sites / Philippe Vain and Vera Thole -- 14. Fluorescent In Situ Hybridization to Detect Transgene Integration into Plant Genomes / Trude Schwartzacher -- 15. Establishing Substantial Equivalence: Transcriptomics / Maria Marcela Baudo, Stephen J. Powers, Rowan A. C. Mitchell and Peter R. Shewry -- 16. Establishing Substantial Equivalence: Proteomics / Alison Lovegrove, Louise Salt and Peter R. Shewry -- 17. Establishing Substantial Equivalence: Metabolomics / Michael H. Beale, Jane L. Ward and John M. Baker -- 18. Design and Management of Field Trials of Transgenic Cereals / Zoltan Bedo, Mariann Rakszegi and Laszlo Lang -- 19. GM Risk Assessment / Penny A. C. Sparrow -- Pt. V. Conclusions -- 20. Transgenic Wheat, Barley and Oats: Future Prospects / Jim M. Dunwell.

ISBN

• 9781588299611 (alk. paper)
• 1588299619 (alk. paper)

LCCN

2008933591

OCLC

• ocn227922736
• 227922736
• SCSB-5445159

Owning Institutions

Columbia University Libraries